体外震波对家兔组织超微结构早期损伤的实验研究  

Tissue Ultasttructure effects of ESWL on a Model Experimental Biliary Calculi in Rabbits

作　　者：张东生[1] 洪大蓉[1] 金立强[1] 祝纪山[1] 宋继志[1] 王尔慧[1] 樊嘉[1] 刘胜利[1] 刘蕴芝 刘燕梅[1] 

机构地区：[1]南京铁道医学院电子显微镜室,南京铁道医学院胆道外科震波室

出　　处：《南京医科大学学报（自然科学版）》1995年第2期265-269,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：采用电镜技术研究（ｅｘｔｒａｃｏｒｐｏｒｅａｌｓｈｏｃｋｗａｖｅＬｉｔｈｏｔｒｉｐｓｙ，ＥＳＷＬ）对组织超微结构的影响。观察显示：肝窦和肺泡隔毛细血管内皮肿胀及缺损形成、损伤区血小板聚集脱颗粒、中性白细胞滞留。肝细胞线粒体肿胀空化，粗面内质网扩张囊泡化及脱颗粒。肺泡出血水肿。镧标记和ＡＬＰ定位技术证明镧颗粒进入细胞及线粒体、毛细胆管紧密联接区有镧沉积和ＡＬＰ反应物。结果提示：毛细血管内皮可能是震波作用的靶细胞；细胞对震波的早期损伤反应是膜正常通透性的丧失，进而导致细胞及细胞器的肿胀和破坏。Effects on liver and other organs of experimental rabbits with biliary calculi undergoing ESWL were studied on electron microscopy. The endotheliocytes of hepatic sinusoids and alveolar capillaries were swollen and damaged; many aggregated platelets with release of cytoplasmic granules adhered to the damaged endotheliocytes and the neutrophils detained in the lumen of capillaries; there was bleeding in the alveolar capillaries. Changes of liver commonly seen include hydropic mitochondria,frank cavitation of the mitochondria matrix and loss of cristae,dilatation and vesiculation of endoplasmic reticulum. The application of lanthanum as a marker in ultrastructural study demonstrated that intracellular lanthanum could be observed and it entered the swollen mitochondria selectively. With deposition of ALP ase reaction products and lanthanum in the tight junctions of capillaries. The results suggest that the capillary endotheliocytes appear to be the target of shock wave,and injury of the permeability of the membranous organelles is the early effects of ESWL on the tissue.

关 键 词：体外震波碎石 超微结构 硝酸镧 胆结石 

分 类 号：R454.3[医药卫生—治疗学]