对BIV－Gag和Env抗原特异性抗体的免疫检测  

IMMUNO-DETECTION OF SPECIFIC ANTIBODIES TO BIV GAG AND ENV PROTEINS

作　　者：陈荷新[1] 刘淑红[1] 纪永刚[1] 耿运琪[1] 

机构地区：[1]南开大学生命科学学院

出　　处：《南开大学学报（自然科学版）》1995年第3期6-10,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家教委博士点基金

摘　　要：本文用高效表达载体表达出的融合ＢＩＶ核心蛋白（ＴｒｐＥ－Ｇａｇ）和外膜蛋白（ＴｒｐＥ－Ｅｎｖ）作为ＷｅｓｔｅｒｎＢｌｏｔ检测的抗原，分别对Ⅰ、Ⅱ两口岸进口奶牛及后代进行了流行病学调查，发现用外膜蛋白检测阳性率比核心蛋白阳性率略高，进一步的追踪调查表明核心蛋白的抗体水平在减弱的同时外膜蛋白的抗体有加强的趋势。With the high-expressed fusion core-protein trpE-Gag and envelope-protein trpE-Env as the detective antigens,the imported cows with their progenies were detected by Western Blot assay. The result shows that the rate of positive result by using TrpE-Env as the detective antigen is higher than that by using TrpE-Gag as the detective antigen. Tracing survey of positive cattle indicates that the Gag-specific antibodies in infected cattles are lossing,in contrast,the level of Env-specific antibodies is strengthening.

关 键 词：BIV 核心蛋白 外膜蛋白 牛 抗原 抗体 免疫检测 

分 类 号：S858.230.2[农业科学—临床兽医学]