男性生殖系统表皮生长因子受体(EGF-R)的分布及精浆中EGF含量的测定  

STUDIES ON REGULATION OF MALE REPRODUCTION FROM THE RECEPTOR OF EPIDERMAL GROWTH FACTOR (EGF) IN MALE REPRODUCTIVE SYSTEM AND EGF CONTENTS IN SEMINAL PLASMA

作　　者：王春年[1] 褚劲松[1] 徐胜[1] 谢文英[1] 方传梁 

机构地区：[1]上海第二医科大学组胚教研室,200025 [2]上海生殖医学研究中心

出　　处：《男性学杂志》1995年第3期134-136,共3页

摘　　要：本文报道了用免疫细胞化学和放射免疫学方法对男性生殖系统表皮生长因子受体(EGF-R)的分布及精浆中EGF含量的研究。结果表明:(1)在人类睾丸内的精原细胞、精母细胞、精子细胞、精子及支持细胞、附睾上皮细胞、前列腺上皮细胞和射出精子的顶体区、尾部的膜上均有EGF-R存在。(2)正常人精浆中EGF含量为18.2±6.1ng/ml,精子计数正常的不育患者精浆中EGF含量为18.5±7.3ng/ml,两组间无显著性差别(P>0.05)。真性无精症精浆中EGF含量为29.5±12.4ng/ml,与正常组间有显著性差别(P<0.01)。由此可见,EGF可能参与精子发生的调节。同时,EGF的含量也受到精子发生的影响。The epidermal growth factor (EGF) receptor in male reproductive system and EGF content in seminal plasma were performed by using immunocytochemical and radioimmunoassay methods in this paper. The results showed: (1)There are the EGF- receptors on Cell membrane of spermatogonium,spermatocyte, spermatid, sperm, Sertoli cell, epididymis epithelium, prostate gland epithelium. (2)EGF content of normal human seminal plasma is 18. 2 ± 6. 1 ng/ml, of infertile with spermatozoa is 18. 46±7. 3 ng/ml (P>0. 05), of a-zospermatozoa is 29. 5 ± 12. 4 ng/ml (P<0. 01). Therefore,spermatogenesis were regulated by EGF.

关 键 词：表皮生长因子 受体 免疫细胞化学法 男性 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]