盐适应和非盐适应红豆草（OnobrychisviciifoliaScop）愈伤组织蛋白质组分变化  

Change of Proteins from Adapted Callus and Unadapted Callus of Onobrychis viciifolia Scop

作　　者：刘顺先 尹俊[1] 张力峰[1] 达来[1] 

机构地区：[1]内蒙古大学生物学系

出　　处：《内蒙古大学学报（自然科学版）》1995年第4期478-480,共3页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金

摘　　要：通过在培养基逐代增加氯化钠浓度的方法获得耐０．５％、１．０％、１．５％ＮａＣｌ的红豆草愈伤组织细胞系，并分别继代培养３０、２５、２０次，与非盐适应细胞系的蛋白质电泳图谱相比，其蛋白质ＳＤＳ－ＰＡＧＥ图谱中出现了８３ＫＤ、３５ＫＤ和１８ＫＤ蛋白质，而非盐适应细胞系中的７０ＫＤ、３７ＫＤ、３２ＫＤ蛋白质则消失或减少．上述８３ＫＤ、３５ＫＤ、１８ＫＤ蛋白质的诱导产生和红豆草愈伤组织在盐环境中表现出的适应性相伴随发生，表明这三种蛋白质可能与抗盐性有关．ells lines of Onforychis viciifolia Scop which are adapted to grow in medin with concentra-tions of 0.5%、1.0%and 1.5%NaCl are obtained.And are maintained 30、25、20 generations aremaintained respectively. Compared with the electrophogrem of proteins from unadapted cellslines,the 83KD、35KD and 18KD proteins in the SDS-DAGE electrophogrem of proteins arefound.And the 70KD、37KD、32KD proteins of unadapted cell lines are disappeared or have re-duced.The induction of 83KD、35KD and 18KD proteins from adapted cells lines is accompaniedwith the adaptability of Onobrychis viciifolia in salt enviroment.It shows that these proteins maybe involved in salt-adaptability.

关 键 词：红豆草 愈伤组织 蛋白质 盐胁迫 盐蛋白 耐盐性 

分 类 号：Q949.751.9[生物学—植物学]