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作 者:朱培良[1] 沈瑛[1] 袁筱萍[1] 赵新华[1]
机构地区:[1]中国水稻研究所
出 处:《农业生物技术学报》1995年第2期64-68,共5页Journal of Agricultural Biotechnology
基 金:洛氏基金
摘 要:用重复序列探针MGR—586,与限制性内切酶EcoRI组合,分析了我国南方部分稻区86个稻瘟病菌株的限制性片段长度多态性(RFLPs)依其MGR—DNA指纹的相似性,结合病菌的致病型测定,将表现为28个不同致病型的86个稻瘟病菌株区分为18个系谱,每个系谱的寄主范围有限,且显示出不同栽培稻区内稻瘟病菌的群体结构有明显差别。本研究通过分析一稻区内稻瘟病菌的群体遗传结构,用分子生态学的方法探索病流行的宏观规律,是分子植物病理学中研究病害流行的新领域。The MGR DNA fingerprints of ca, 86 isolates of M,grisea in the partial rice growing ar-eas of southern China have been studied with a dispersed repeated sequence probe MGE 586/lEcoRI digestcombination,The fingerprint data document a generalized genetic organization。Twenty eight pathotypesof M,grisea can be distinguished into 18 lineages based on MGR DNA fingerprint similarity.Each lin-eage has a limitted host cultivar range and is assocoated with one to several related pathotypes,and this re-lationships between and among pathotypes are maintained over several years across broad geographicdistribution. The genetic structure of M. grisea population could be analysed further in one rice growingarea according to the present study.The macroscopic law of the epidemic disease to rice blast was studiedby molecular ecological methods. It is the new research area to study the disease epidemics in molecularplant pathology.
分 类 号:S435.111.4[农业科学—农业昆虫与害虫防治]
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