转CP基因的番茄、南瓜和甜瓜植株的抗病性研究  

Study on Resistance to CMV in Transgenic Tomato,Squash and Muskmelon Plants that Express the Cucumber Mosaic Virus Coat Protein Gene

作　　者：薛宝娣[1] 陈永萱[1] 

机构地区：[1]南京农业大学植病系,康乃尔大学农业试验站

出　　处：《农业生物技术学报》1995年第2期58-63,共6页Journal of Agricultural Biotechnology

摘　　要：借助土壤农杆菌Ｔｉ质粒介导的叶盘转化法获得了番茄、南瓜三种作物的黄瓜花叶病毒外壳蛋白（ＣＭＶ—ＣＰ）基因工程植株。比较了三种转化植株对ＣＭＶ的抗病性类型。试验结果：番茄转化植株表现为免疫反应，南瓜为抗扩散反应，而甜瓜主要表现为早期抗性，即明显推迟接种植株的发病。用二个不同血清型的ＣＭＶ株系接种三种作物的转化植株，同一种转化植株对不同株系的抗性反应一致。三种转化作物中表达了ＣＭＶ—ＣＰ基因的植株均能抗ＣＭＶ强毒株系的侵染。番茄和南瓜转化植株的抗病程度与植株中的ＣＰ含量无相关性。Transgenic tomato,squash,muskmelon plants were obtained using the agrobacteriuntmefaciens methods.The transferrd genes were from the T-DNA region of the binary vector plasmid PGA482GG/CP-CMV,The three plants that express the CMV-CP gene were challenge-inoculated with CMVrespectively. Resistance to CMV varied accoding to the three different transgenic plants,Tomato plants ex-pressing CMV-CP gene showed immune infection;squash plants showed resistance to the virus diffusion;and the transgenic muskmelon plants showed mainly a delay in symptom development.the three plants ex-pressing the CMV-CP gene were also inoculated with CMV stains belong to two CMV subgrorp.They ap-peared to show a broad spectrum of protection against infection by the various CMV stains. In the com-pared experiment with transgenic tomato and squash plants,CP Ievels of transgenic plants dld not showreletion to the resistance to the CMV infection.

关 键 词：番茄 南瓜 甜瓜 转基因植株 抗病性 

分 类 号：S332.2[农业科学—作物遗传育种]