玉米胚性愈伤组织转化及转Bt基因植株的抗虫性  

Transfer of Bt-toxin Protein Gene into Maize and the Resistance of Transgenic Plants to Corn Borer

作　　者：王国英[1] 张宏[1] 谢友菊[1] 戴景瑞[1] 米景九 李太源[1] 田颖川[1] 乔利亚[1] 莽克强[1] 

机构地区：[1]北京农业大学生物学院,中国科学院微生物所

出　　处：《农业生物技术学报》1995年第3期49-53,共5页Journal of Agricultural Biotechnology

基　　金：863资助项目

摘　　要：用基因枪直接轰击玉米胚性愈伤组织，经潮霉素抗性选择平均每皿可得到０．７～２．０块抗性愈伤组织，且大部分可再生植株。对这些从抗性愈伤组织分化的植株进行点杂交和Ｓｏｕｔｈｅｒｎ吸印杂交的结果也证明Ｂｔ基因已插入玉米染色体组。对一个转Ｂｔ基因植株的测交后代进行了玉米螟抗性的田间鉴定，抗虫和感虫植株的比例接近１：１，符合孟德尔单显性基因的遗传规律。Maize embryogenic calli were bombarded by high- velocity microprojectiles coated withplasmid pB48.4l5 DNA, which contained Bt-toxin protein gene and hygromycin phosphotransferase gene.After being selected on medium with hygromycin the resistant embryogenic calli were transferred to regen-eration medium on which embroids formed and germinated.The results of dot blotting and Southern hy-bridization certified the integration of Bt-gene into maize genome. The prelirninary results of insect resis-tance evaluation on the progenies from one Bt-transgenic plant showed that the ratio of resistant and sus-ceptible plants was near to l:l.

关 键 词：玉米 转化 抗虫性 愈伤组织 

分 类 号：S513.035.3[农业科学—作物学]