GUS基因和CMV-CP基因在转基因番茄后代的遗传研究  

Inheritance and Stable Expression of GUS Gene and CMV-CP Gene in Transgenic Tomato

作　　者：薛宝娣[1] 陈永萱[1] 汪智渊[2] 吴汉章[2] 

机构地区：[1]南京农业大学植保系,南京210095 [2]江苏丘陵地区镇江农科所

出　　处：《农业生物技术学报》1995年第4期84-88,共5页Journal of Agricultural Biotechnology

摘　　要：本工作对外源基因 GUS 和 CMV-CP 在转化番茄植株后代的遗传稳定性方面作了研究。通过对 GUS 基因在转化番茄 R_1～R_5代植株中的活性测定和 GUS 与 CMV-CP 二个基因在 R_1代植株中的检测,表明:R_1、R_2代的 GUS 基因为1:1分离。R_3和 R_4代的植株 GUS 基因出现了3:1单基因显性遗传规律,1:1分离单株比例逐渐减少。从 R_3和 R_4代的单株 GUS 基因出现了3:1单基因显性遗传规律,1:1分离单株比例逐渐减少。从 R_5代中选择到2个 GUS基因纯合的单株,得到了遗传性稳定的单株,说明 GUS 基因在转化番茄植株后代的遗传应属于单基因显性遗传规律。实验证明,位于同一质粒上的 GUS 和 CMV-CP 基因,在 R_1代番茄转化植株中表现为连锁遗传。Inheritance and stable expression of foreign genes GUS and CMV-CP in transgenic tomato was studied,and the transgenic tomato was obtained by transforming agrobacterium tumefacience with GUS and CMV-CP genes residing on the Ti plansmid constructs.Inheritance of the transgenes in R_1 gener- ation of the rangenic tomato was investigated by enzyme assays and DAS-ELISA,the results showed that the two transgenes resided on the plasmid were co-inherited as a single inhertance unit.Analysis of GUS activities in R_1 up to R_5 generation tested provided that the segretion of the GUS gene in the R_1 and R_2 gen- eration was 1:1,and the inheritance in R_3 to R_5 was in mendelian fashion(3:1).Two homozygous line expressing the GUS were obtained in R_5 generation.

关 键 词：转基因番茄 GUS基因 CMV-CP基因 遗传 

分 类 号：S641.203.2[农业科学—蔬菜学]