对虾体内氯霉素含量测定方法的研究  

STUDIES ON THE METHOD OF DETERMINATION OF CHLOROMYCETIN CONTENT IN PENAEUS CHINENSIS

作　　者：李兰生[1] 王勇强[1] 

机构地区：[1]青岛海洋大学,山东省海水养殖研究所

出　　处：《青岛海洋大学学报（自然科学版）》1995年第3期400-406,共7页Journal of Ocean University of Qingdao

摘　　要：应用ＨＰＬＣ技术建立常用药物氯霉素的虾体动力学研究方法。该方法的特点是：与微生物法、比色法、气相色谱法、荧光法及免疫法比较．测试准确、快速且灵敏度高。应用该方法可以从虾体内的血液、肌肉、肝胰脏、肠胃中分离出被虾吸收的氯霉素，并定量测定。在１—５０μｇ／ｍｌ范围内线性关系相关系数为０．９９９５。A method for pharmacolinetic study on antibiotic chloromycetin in Penaeus chinensis body was established by HPLC. It is more rapid, accurare and sensitive than microbiological, colorimetric analysis, gas chromatography, fluorescence analysis and the enzyme method to separate and determinate quantitatively chloromycetin from blood, muscle, hapato--pancreas, intestineswstomach of penaeus chinensis. The correlation coefficient is 0. 9995 in the range 1-- 50μg/ml.

关 键 词：对虾 体内 氯霉素 测定法 液相色谱仪 

分 类 号：Q959.223.5[生物学—动物学]