高效液相色谱法同时测定强化牛奶中的维生素A和D_2  

Simultaneous Determination of Vitamin A and D_2in Fortified Milk by High Performance Liquid Chromatography

作　　者：赵慧芬[1] 李克杰[1] 曹燕叔[1] 郑建国[1] 

机构地区：[1]北京奶牛中心

出　　处：《色谱》1995年第1期61-62,共2页Chinese Journal of Chromatography

摘　　要：高效液相色谱法同时测定强化牛奶中的维生素Ａ和Ｄ＿２赵慧芬，李克杰，曹燕叔，郑建国（北京奶牛中心北京１０００８５）１前言维生素Ａ，Ｄ＿２（ＶＡ，ＶＤ＿２）是人体所必需的营养物质。强化ＶＡＤ消毒牛奶的出现，需要建立快速准确的分析方法。用高效液相色谱法（Ｈ...reversed-phase high performance liquid chromatographic method for the simultaneous determination ofvitamin A and D_2 in fortified milk has been developed. A couple of serially connected columns with μ-Bonda-pak C_(18)(300 × 3. 9mm, 10μm)and Novapak C_(18)(150 × 3. 9mm, 4μm)and methanol/water(96/4)mobile phasewere used. Vitamin A and D_2 were detected at wavelengths of 325nm and 265nm, respectively. By usingsaponification procedure, the recoveries of vitamin A and D_2 were in the range of 90～110%, and the coeffi-cients of variation were lower than 8%.

关 键 词：HPLC 牛奶 VA VD 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] O652.63[轻工技术与工程—食品科学与工程]