固相萃取和毛细管气相色谱－红外光谱联用分析生物样品中的七种巴比妥类药物  

Analysis of Seven Barbiturates in Biological Materials by Solid-PhaseExtraction and Capillary Gas Chromatography with FTIR Detection

作　　者：张喜轩[1] 范垂昌[1] 贾静涛[1] 

机构地区：[1]中国医科大学法医学系

出　　处：《色谱》1995年第3期182-184,共3页Chinese Journal of Chromatography

摘　　要：报告了一种新的巴比妥类药物的分析方法，１ｍＬ血浆或尿（含各药１０μｇ）用稀盐酸稀释至１０ｍＬ，离心１０ｍｉｎ，慢慢通过Ｃ＿（１８）柱。用稀盐酸洗Ｃ＿（１８）柱２～３次，用３ｍＬ氯仿／甲醇洗脱药物，洗脱液在氮气流中吹干，用５０μＬ酸性甲醇溶解残渣，取１μＬ用于气相色谱分析。用傅立叶变换红外光谱仪检测气相色谱图，得到了较好的结果。血浆中药物检测限低于１μｇ／ｍＬ。n t his Paper, a new method for the analysis of seven barbiturates in biological materials is reported.One milliliter of plasma(or urine)which contained 10 μg of each barbiturate was mixed with 9mL of diluteHCl. The mixture, after centrifugation for about 10min, passed a C_(18) cartridge slowly. The cartridge waswashed for 2 to 3 times with dilute HCl and a 3mLof chloroform/methanol(9:1)solution was used to elutethe drugs from the cartridge. The eluate was dried out under nitrogen stream and the residue was dissolved in50μL of acidic methanol. One microliter was taken for capillarygas chromatographic analysis. Fourier Trans-form Infrared Spectrometry was employed to detect the chromatograms. The results were very satisfactoryand the detection limit in plasma was below 1μg/mL.

关 键 词：毛细管气相色谱 巴比妥 红外光谱检测 血浆 尿 

分 类 号：R971.3[医药卫生—药品] O657.71[医药卫生—药学]