气相色谱法用于分枝杆菌脂肪酸组成与菌种鉴定的研究  

Gas Chromatographic Analysis of Cellular Fatty Acids of Mycobacteriums

作　　者：康建[1] 马小锋[1] 包美复 侯晓娜[1] 

机构地区：[1]沈阳军区总医院,鞍山市千山医院

出　　处：《色谱》1995年第4期269-271,共3页Chinese Journal of Chromatography

摘　　要：采用非极性毛细管柱气相色谱法对３株分枝杆菌细胞脂肪酸化学组成进行了研究，得到特异性指纹识别图，并对７例临床分离的分枝杆菌进行了鉴别。结果表明，结核菌硬脂酸（ＴＳＡ）为分枝杆菌属的特征组分，其含量与其他中长链脂肪酸的百分组成随菌种不同而异。he fatty acids of 3 strains of various mycobacteriums and 7 strains of mycobacteriums tuberculosis iso-lated from naturally infected patient fluids were studied by capillary gas chromatography.All strains con-tained the characteristic mycobacterial liquid constituent, tuberculostearic acid.Contents of tuberculostearicacid and other fatty acids were different with various mycobacteriums. The fatty acid patterns and especiallythe presence of tuberculostearic acid are characteristic marker of mycobacterium,Gas chromatography mayprovide a convenient alternative to microorganism culture assay.

关 键 词：气相色谱 分枝杆菌 脂肪酸 结核菌硬脂酸 

分 类 号：R378.911[医药卫生—病原生物学] O657.71[医药卫生—基础医学]