细粒棘球蚴实验感染鼠体液免疫动态的初步结果  

IMMUNOLOGICAL HUMORAL RESPONSE IN MICE EXPERIMENTALLY INFECTED WITH ECHINOCOCCUS GRANULOSUS, PRELIMINARY RESULT OF KINETIC OBSERVATION

作　　者：薛海筹[1] 裘丽姝[1] 张永红[1] 张超威[1] 朱震霞[1] 杨元清[1] 陆健 李浩[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1989年第3期170-173,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：采用胶乳凝集试验(LA)、酶联免疫电转移印斑试验(EITB)、酶联免疫吸附试验(ELISA)、斑点-ELISA试验(Dot-ELISA)以及免疫荧光试验(IFA)5种免疫血清学方法,对细粒棘球蚴实验感染鼠的抗体进行了动态观察,结果表明这5种检测方法均能检出抗体,其中以Dot-ELISA及IFA最敏感,于感染后2周即有部分鼠测到抗体。ELISA、EITB和LA出现阳性反应的时间依次为4周、2月和3月。感染8月及1年的病鼠全部阳性,而对照鼠则全部阴性。5 serological methods included latex agglutination test (LA), enzyme-linked immu-noelectrotransfer blot (EITB), enzyme-linked immunosorbent assay(ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA) and immunofluorescence antibody test (IFA) were used to trace tne kinetic changes of the humoral response in NIH mice experimen-tally infected with protoscolex of Echinococcus granulosus. Results showed that Dot-ELISA and IFA were able to detect antibodies 2 wk after infection while ELISA, EITB and LA gave positive reactions 4 wk, 2 mos and 3 mos after infection respectively. Therefore Dot-ELISA and IFA were considered to be the most sensitive ones among the 5 methods used. All infected mice gave positive reactions at 8 and 12 mos but none of the normal control mice showed any reaction throughout the period of observation (Fig. 1).

关 键 词：包虫病 细粒棘球蚴 抗体 

分 类 号：R532.8[医药卫生—内科学]