免疫酶染色试验及双面胶纸条法环卵沉淀试验诊断血吸虫病的比较  

COMPARISON OF IMMUNOENZYMATIC STAINING TECHNIQUE (IEST), DOUBLE GLUING STRIP CIRCUMOVAL PRECIPITIN TEST(DGS-COPT) AND CONVENTIONAL CIRCUMOVAL PRECIPITIN TEST(CV-COPT) FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

作　　者：李允鹤[1] 胡永德[1] 许文美 杨光堡 殷水龙 龚唯[1] 朱家勇[2] 吴宗泉[1] 骆伟[1] 

机构地区：[1]苏州医学院寄生虫学教研室 [2]苏州医学院寄生虫学专业

出　　处：《中国寄生虫学与寄生虫病杂志》1989年第4期263-266,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金："七.五"国家攻关课题~~

摘　　要：应用免疫酶染色试验(IEST)及双面胶纸条法COPT(DGS-COPT),并以常规法DOPT(CV-COPT)为对照,采用单盲法比较检测120例血吸虫病人。120例经吡喹酮治疗后3～4年及≥5年的血吸虫病人和120名健康人的血清。三种方法的敏感性和特异性:IEST分别为91.7和95.8％;两种COPT分别为81.7～82.5和96.7～97.5％,无显著性差异。治疗后3～8年血吸虫病人的阴转率分别为70.8％(IEST)、80.8%(DGS-COPT)和81.7%(CV-COPT)。表明IEST的诊断效果显著地高于两种COPT,并可弥补COPT漏检病人的缺陷。IEST, DGS-COPT and CV-COPT using lyophilized ova of schistosoma japonicum were performed on sera from 120 cases of schistosomiasis japonica, 120 cases of schis-tosomiasis japonica 3-8 years after being cured with praziquantel and 120 healthy individuals by single-blind method. The sensitivity and specificity of IEST was 91.7% and 95.8% respectively which were significantly higher than that of both DGS-COPT and CV-COPT. The negative conversion rate of cured patients was 70.8% with IEST, 80.8% with DGS-COPT and 81.7% with CV-COPT. The results showed that IEST has higher diagnostic value for schistosomiasis than both COPT. DGS-COPT has the same diagnostic value as CV-COPT, however, it was easy to perform and time-saving, thus it might be applied in the fields for practical purposes.

关 键 词：免疫酶染法 血吸虫病 环卵沉淀 

分 类 号：R532.21[医药卫生—内科学]