MPTP对DA、GSH和LPO浓度的影响及Deprenyl的干预  

Effects of MPTP on the Levels of Dopamine,Glutathione and Lipid Peroxidation and prevention of Deprenyl Against the Neurotoxicity og MPTP

作　　者：陈生弟[1] 刘振国[1] 余慧贞[1] 唐琴梅[1] 徐修容[1] 

机构地区：[1]上海第二医科大学附属瑞金医院神经内科,中国科学院上海药物研究所

出　　处：《上海第二医科大学学报》1995年第1期1-4,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：运用高效液相色谱－电化学（ＨＰＬＣ－ＥＣ）法、二硫双硝基苯甲酸（ＤＴＮＢ）比色法和硫代巴比妥酸（ＴＢＡ）反应法分别检测多巴胺（ＤＡ）、谷胱甘肽（ＧＳＨ）和丙二醛（ＭＤＡ）含量，研究神经毒素甲基－苯基－四氢吡啶（ＭＰＴＰ）对这些物质含量的影响，以及单胺氧化酶Ｂ抑制剂ｄｅｐｒｅｎｙｌ对ＭＰＴＰ的干预作用。结果表明，ＭＰＴＰ具有显著降低ＤＡ、ＧＳＨ含量，以及增加ＭＤＡ含量的作用，ｄｅｐｒｅｎｙｌ可完全抑制ＭＰＴＰ的上述作用。The effects of methyl-phenyl-tetrahydropyridine (MPTP) on the levels of dopamine(DA), glutathione (GSH) and malonaldehyde (MDA)were studied.Following MPTP intoxication, the contents of DA and GSH were remarkably reduced as showen by high performance liquid chromatography with electrochemical detection and dithionitrobenzoic acid (DTNB) determination, while the concentration of MDA was elevated by thiobarbituric acid (TBA) reaction. Deprenyl,a B-type monoamine oxidase inhibitor, can completely prevent against the neurotoxicity of MPTP.

关 键 词：神经毒素 MPTP DEPRENYL 震颤性麻痹 DA GSH 

分 类 号：R994.3[医药卫生—毒理学] R742.502[医药卫生—药学]