恶性疟原虫113肽抗原基因重组的减毒鼠伤寒沙门菌在家兔中免疫应答的研究  

Antigenicity of Recombinant S. typhimurium Expressing a Hybrid 113peptide Antigen of Plasmodium falciparum in Rabbits

作　　者：黄建生[1] 王昌才[1] 任大明[1] 李全贞[1] 钟雄林[1] 

机构地区：[1]广州第一军医大学分子生物学研究所,上海复旦大学遗传所国家重点实验室,广州第一军医大学热带医学研究所

出　　处：《上海免疫学杂志》1995年第5期269-272,共4页Shanghai Journal of Immunology

摘　　要：我们已经在减毒鼠伤寒沙门菌ＳＬ３２６１以融合蛋白的形式表达了人工合成的恶性疟原虫杂合１１３肽基因ＡＢ（ＧＺ－ＡＢ）。活菌以２×１０９ＣＦＵ经口服免疫新西兰家兔，用ＥＬＩＳＡ测定抗体水平，结果于首次免疫或加强免疫后都可检测到一定水平的特异性抗体。所免疫的家免可以诱发针对恶性疟原虫抗原及ＧＺ－ＡＢ的迟发性超敏反应（ＤＴＨ）。我们的研究表明，含有多个恶性疟原虫抗原表位的人工合成基因可以在减毒鼠伤寒沙门菌中表达，活菌可诱发家兔产生特异的体液免疫及细胞免疫，为恶性疟口服活菌苗的制备打下基础。We have expressed a 113-peptide hybrid antigen of Plasmodium falciparum as fusion protein in attenuated Salmonella typhimurium SL3261. Live recombinant vaccine was orally immunized rabbits with a dose of 2×109 CFU.Specific anti-serumcould be detected by ELISA after immunization.Obvious delayed type of hypersensitivity(DTH)could be induced by Pf-Ag and GZ-AB antigens. Our studies indicatethat the synthetic genes encoding several antigenic epitopes of P.falciparum couldbe expressed in the attenuted S.typhimurium, and live organisms could induce special cell-mediated and humoral immunity,which preliminarily prove the possibility ofP. falciparium oral live vaccine.

关 键 词：鼠伤寒沙门氏菌 恶性疟原虫 活菌苗 

分 类 号：R392-33[医药卫生—免疫学] R978.61[医药卫生—基础医学]