抗人心肌肌球蛋自重链单克隆抗体的筛选与鉴定  

Production and Characterization of Monoclonal Antibody Directed against Human Cardiac Myosin Heavy Chain

作　　者：邹立华[1] 杨一峰[1] 顾宇参[1] 钱忠豪[1] 陈灏珠[1] 孙燮钧 

机构地区：[1]上海市心血管病研究所,中国科学院上海细胞生物学研究所,新疆医学院第一附属医院心内科

出　　处：《上海免疫学杂志》1995年第6期337-339,共3页Shanghai Journal of Immunology

基　　金：高校博士学科点专项科研基金

摘　　要：用自制人心肌肌球蛋白作为哺抗原，按杂交瘤常规技术获得一株稳定的Ｇ４杂交瘤细胞株。单抗亚型为ＩｇＧ１型；用免疫印迹法对成人、胎儿、哺乳类动物及鲫鱼的心肌肌球蛋白提取液在２００ｋＤ处有一着色带；免疫组化ＡＢＣ法表明单抗与成人、胎儿、猪、兔、大白鼠的心肌细胞胞浆均有阳性反应，与血管平滑肌均呈阴性反应。该单克隆抗体可用于与心肌坏死病理过程有关的疾病的诊断。Balb/c mice were immunized by injection of human cardiac myosin extracted by ourselves. By the routine hybridoma techniques, a stable G4 specific hybridoma cell line was obtained. This monoclonal antibody was detected to be IgG1. Western blot analysis for antigen detection using specific anti-myosin heavy chain McAb showed a band at 200kD position which included human, fetus and other animal cardiac myosins. Immunohistochemistry studies with ABC method indicated that left ventricular specimens from above cardiac tissues showed cardiac cytoplasms in which myosin existed were positively stained. In contrast, arterial blood vessels appeared to be negatively stained. The results suggested that this monoclonal antibody may be used for th diagnosis of those diseases related to the pathologic courses of myocardial necrosis.

关 键 词：肌球蛋白 重链 单克隆抗体 制备 鉴定 

分 类 号：R392－33[医药卫生—免疫学]