维生素氨基酸对石刁柏愈伤组织生长和多糖含量的影响  

EFFECTS OF VITAMINS AND AMINO ACIDS ON GROWTH AND POLYSACCHARIDE AMOUNT IN Asparagus officinalis L.

作　　者：陆仕华[1] 蒋琳[1] 周正来[1] 

机构地区：[1]上海市农业科学院植物保护研究所

出　　处：《上海农业学报》1995年第1期87-90,共4页Acta Agriculturae Shanghai

摘　　要：报道了３种维生素（Ｂ６、烟酸、肌醇）和４种氨基酸（精氨酸、谷氨酸胺、天冬酰胺和天冬氨酸）对石刁柏愈伤组织生长和多糖含量的影响。烟酸（１．０ｍｇ／Ｌ）对愈伤组织生长和多糖含量的提高均有促进作用，肌醇（２００ｍｇ／Ｌ）对多糖含量提高较显著，Ｂ６（１．０ｍｇ／Ｌ）对生长和多糖含量的提高有抑制作用。烟酸浓度以２ｍｇ／Ｌ最佳。４种氨基酸对生长和多糖含量提高均有促进作用。其中精氨酸（１０～２０ｍｇ／Ｌ）和谷氨酸胺（１０ｍｇ／Ｌ）对生长和多糖含量的提高均有促进作用。当浓度＜５ｍｇ／Ｌ时，谷氨酰胺促进生长作用稍低于精氨酸；当浓度≥１０ｍｇ／Ｌ时，谷氨酸胺促进生长和提高多糖的作用远强于精氨酸。从２８个无性系中初步选出Ｎｏ．６和Ｎｏ．２５二株，它们生长快且含多糖量最高，可供进一步试验。it was reported that 3 vitamins(B6, nicotinic acid and inositol) and 4 amino acids(Arg, Asp, Asn and Gln) affected the growth and polysaccharide amount in asparagus calli. Nicotinic acid (10mg/L) could promote increase in growth and polysaccharide amount of calli, and inositol (200mg/L) increased the polysaccharide amount greatlyl however, B6 (1. 0mg/L)showed inhibitory effect on both growth and polysaccharide amount. The optimun concentration of nicotinic acid was 2mg/L. All 4 amino acids promoted both growth and polysaccharide amount especially Arg (10￣ 20mg/L) and Gin (10mg/L). At concentration less than 5 mg/L, Gin promoted the growth slightly lower than Arg did, however at concentration increased to 10mg/L or more,it promoted the increase in growth and polysaccharide amount much stronger than Arg. The clones No. 6 and No. 25 were selected from 28 clones for further researches, The two clones with good morphology grew rapidly and their average polysaccharide amount 17. 5% was more than that of the middle part of asparagus stem in fields.

关 键 词：石刁柏 愈伤组织 烟酸 精氨酸 谷氨酰胺 多糖 

分 类 号：S644.601[农业科学—蔬菜学]