植物病害抗性基因克隆策略  

STRATEGIES FOR THE CLONING OF PLANT GENES CONFERRING RESISTANCE TO PATHOGENS

作　　者：姚泉洪[1] 黄晓敏[1] 刘宗镇[1] 蒋玲 戴富民[1] 

机构地区：[1]上海市农业科学院植物保护研究所

出　　处：《上海农业学报》1995年第2期91-96,共6页Acta Agriculturae Shanghai

摘　　要：现代分子遗传学的迅速发展对分离和鉴定植物病害抗性基因起了积极推动作用。迄今植物病害抗性基因克隆的策略主要有：“鸟枪”克隆法，植物转座子示踪与Ｔ－ＤＮＡ插入突变法，ＲＦＬＰ和染色体步移法，纯化病原菌特异致激物受体法。本篇综述旨在介绍这些抗病基因的克隆策略及研究进展。The rapid development of molecular genetics plays an active promoting role in identifying and isolating genes that upon transfer may render target plants resistant to fungal pathogens. To date, several gene cloning strategies have been proposed. These include 'Shotgun' cloning, cloning by transposon tagging or T-DNA insertional mutagenesis, RFLP and chromosome walking and cloning of genes encoding receptors for race-specific elicitors. The objective of this review is to provide a summary of the current researches on these strategies.

关 键 词：植物病害 抗病基因 基因克隆 

分 类 号：S432[农业科学—植物病理学]