用高专属性酶分析法测定果糖─1，6－二磷酸及其水针剂的稳定性  

DETERMINATION OF FRUCTOSE-1,6-DIPHOSPHATE(FDP)AND THE STABILITY OF 10% FDP SOLUTION FOR INFUSION BY A HIGHLY SPECIFIC ENZYMATIC ASSAY

作　　者：段更利[1] 李丽珍[1] 郭仲秋[1] 顾丽华[1] 张莉[1] 佟莱阁 程务本[1] 

机构地区：[1]上海医科大学药物分析教研室,上海市药品检验所,上海职工医学院

出　　处：《上海医科大学学报》1995年第5期341-344,共4页Journal of Fudan University(Medical Science)

摘　　要：利用酶分析法测定果糖－１，６－二磷酸（ＦＤＰ）及其水针剂的稳定性。结果：Ｙ＝０．００８＋１．１０３ｃ，ｒ＝０．９９９８（ｎ＝３）：准确度为９９．９９±２．５７％（ｎ＝５）：天内、天间的ＲＳＤ分别为０．７０％和１．１０％（ｎ＝４）：且不受葡萄糖、果糖的干扰；经４０℃加速试验，强光照射试验及高温试验，ＦＤＰ水针剂的稳定性均好。结论：本法的准确度高、专一性好；我们试制的ＰＤＰ水针剂稳定性好，具有应用价值，值得开发。An enzymatic assay was studied to determine fructose-1, 6-diphosphate(FDP)and the stability of 10%FDP solution for infusion. Results:Y= 0. 008+1.103c,r= 0.999 8(n=3);The accuracy was(99.99±2.57)%(n=5); The RSD of day-to-day and within day were 0. 70% and 1.10%(n=4) ,respectively;Both glucose and fructose did not interfere with the determination of FDP; the 10% FDP solution for infusion had good stability in the tests at 40℃,60℃and in the strong light.These results indicate that this assay is highly accurate and specific and 10% FDP solution for infusion prepared by ourselves has both good stability and use value, so it is valuble to be developed.

关 键 词：果糖 二磷酸 稳定性 水针剂 高专属性酶分析 

分 类 号：R972[医药卫生—药品]