钾离子对体外培养中枢神经髓鞘形成的影响──一种新的体外培养中枢神经脱髓鞘模型的建立  

THE INFLUENCE OF POTASSIUM ION ON THE MYELINATION OF CENTRAL NERVOUS SYSTEM IN VITRO ──A NEW MODEL OF DEMYELINATION OF CNS IN VITRO

作　　者：姜文跃[1] 沈馨亚[1] 苏清芬[1] 彭裕文[1] 汪洋[1] 郑思竞[1] 

机构地区：[1]上海医科大学解剖学教研室

出　　处：《神经解剖学杂志》1995年第1期1-6,共6页Chinese Journal of Neuroanatomy

摘　　要：本文应用组织培养和电镜等方法研究了ＫＣＩ对体外培养中枢神经系统髓鞘形成的影响。结果表明：体外培养Ｂ６Ｃ３小鼠小脑组织髓鞘形成的关键期是１０～１４ｄ，在１０～１２ｄ时体外培养的小脑组织对ＫＣＩ最为敏感；当培养液内ＫＣＩ浓度达到３０ｍｍｏｌ／Ｌ时，即可完全抑制髓鞘形成。本文对钾离子引起体外培养中枢神经组织脱髓鞘的机制进行了讨论。It has been known that the potassium ion (K+) serves as the medium of coordinating signals among among and glias,but how the K+ can influence the myelination of central nervous system (CNS) in vitro is not clear. In order to get further evaluation,CNS tissue culture in K+-containing media as well as LM/TEM has been used in this experiment. The results showed that the key period of myelination in newborn B6C3 mouse cerebellum was 10～14 days in vitro (div), and it seemed most sensitive while in high K+ in 10～12 div; as the concentration of K+ in medium was elevated to 25 mmol/L, the Percentage of myelination was significantly decrease to 16. 67 % (P<0.0001 ), while the shape of myelin sheath was characterized by beaded. The myelination was totally suppressed while concentration of K+ was enhanced to 30 mmol/L, and the morphological properties of neurons were kept normal even in high K+ media. So, the model of demyelination of CNS in vitro can be set up by use of present techniques. The advantageS of the model are as followsl (1)the procedures of the experiment is stablel (2)lower toxicity to neurons and glias; (3)the doSage of agents used to be easily controlled. The mechanisms of demyelination of CNS in vitro in high K+medium are discussed in this paper.

关 键 词：脱髓鞘 体外培养 钾离子 中枢神经系统 髓鞘 

分 类 号：R338.2[医药卫生—人体生理学] Q426[医药卫生—基础医学]