雪旺氏细胞从冷冻人胎周围神经的分离技术及其特性  

ISOLATION AND CHARACTERISTICS OF SCHWANN CELLS DERIVED FROM CRYOPRESERVED HUMAN FETAL PERIPHERAL NERVES

作　　者：严恒林[1] 沈馨亚[1] 刘材栋 陈丽琏[1] 汪洋[1] 王自美[1] 

机构地区：[1]上海医科大学解剖学教研室

出　　处：《神经解剖学杂志》1995年第4期307-314,共8页Chinese Journal of Neuroanatomy

摘　　要：本研究取１５～２０周人胎周围神经，放在冷冻的含ＤＭＥＭ（４０％）、ＤＭＳＯ（１０％）及小牛血清（５０％）中。细胞活性为９１％。雪旺氏细胞经过分离、培养、保持了活性。经３～６月液氮保存后，细胞活性为９４％，雪旺氏细胞在４℃下经２４小时，活性仍有９２％。从新鲜周围神经取得的和经冷冻保存的以及单个培养的雪旺氏细胞，在培养时生长缓慢，但在培养液中加牛脑垂体提取液后，细胞增殖加快。取自新鲜或冷冻的周围神经的雪旺氏细胞用Ｓ－１００蛋白抗体染色的特性相似。本文还报告了分离出的雪旺氏细胞在扫描及透射电镜下的形态特征。The peripheral nerves of human fetus (15～20 week)were placed in freeaing solution containing DMEM(40%), DMSO (10%) and calf serum (50% ) and stored in liquid nitrogen for 3 ～ 6months. The frozen nerves were rapidly thawed to 37℃. Cellular viability was 91 %. Schwann cells detived from. frozen nerves were retained growth by dissociated and explant culture. Moreover cell suspension of Schwann cells were stored in liquid nitrogen for 3～6 months,the cellular viability was 94%, when Schwann cells were kept at 4℃, viability was reduced with time and kept 92% within 24 h at 4℃. Schwanncells from frozen and fresh peripheral nerves as well as single cell suspension grew very slowly in vitro,when bovine pituitary extract was added to medium, Schwann cellsrapidly proliferated.Immunocytocbemical staining of Schwann cells from both frozen-and fresh peripheral nerves produced similar staining patterns with antibody against S-100 protein. Further observation of Schwann cells were also taken under scanning and transmission electron microscopes in order to display its typical morphologicalcharacteristics.

关 键 词：冷冻 周围神经 雪旺氏细胞 人胎 分离技术 解剖 

分 类 号：R322－33[医药卫生—人体解剖和组织胚胎学]