糖皮质激素及其他甾体激素对大鼠下丘脑薄片释放精氨酸加压素的影响  

EFFECT OF GLUCOCORTICOIDS AND OTHER STEROIDS ON ARGININE VASOPRESSIN RELEASE FROM RAT HYPOTHALAMIC SLICES

作　　者：刘秀[1] 王春安[1] 叶阿莉[1] 江萍[1] 陈宜张[1] 

机构地区：[1]第二军医大学生理教研室,江西宜春卫生学校生理教研室

出　　处：《生理学报》1995年第2期201-206,共6页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：本工作采用离体孵育技术，观察大鼠下丘脑薄片（含有室旁核和视上核）释放精氨酸加压素（ＡＶＰ）和糖皮质激素（ＧＣ）及其他甾体激素对ＡＶＰ释放的快速影响。结果如下：（１）大鼠下丘脑薄片经过９０ｍｉｎ的恢复之后，在长达６ｈ的孵育过程中能够相当稳定地释放ＡＶＰ，释放量为９．０６±１．２３ｐｇ／ｍｉｎ；（２）皮质酮（Ｂ）在２０ｍｉｎ内可明显地抑制ＡＶＰ的释放，在１０－７—１０－４ｍｏｌ／Ｌ范围内呈剂量－效应关系；（３）在同一剂量（１０－６ｍｏｌ／Ｌ），皮质醇、１７β－雌二醇和睾丸酮也可快速地抑制ＡＶＰ的释放，而相同剂量的地塞米松、醛固酮、孕酮、ＲＵ４８６和胆固醇却无此效应；（４）ＲＵ４８６（１０－７—１０－３ｍｏｌ／Ｌ）对ＡＶＰ的释放没有影响，但却能（１０－５—１０－３ｍｏｌ／Ｌ）部分地阻断Ｂ的快速抑制效应。这些结果表明，ＧＣ对大鼠下丘脑ＡＶＰ的释放具有不通过传统的基因组机制的快速抑制效应，此种抑制效应可能与ＧＣ的负反馈调节作用有关。Rat hypothalamic slices sectioned with vibratome (containing paraventricular and supraoptic nuclei) were incubated in static microchamber and the Arginine vasopressin (AVP) released from the slices was measured by radioimmunoassay. The effect of glucocorticoids (GC) and other steroids on AVP release was investigated. The results were as follows: (1) AVP was steadily released at a rate of 9. 06 ±1. 23 pg/min for as long as 6 h (not including the 90 min for recovery of slices). (2) Corticosterone (B) inhibited AVP release within 20 min in a dose-dependent manner from 10-7 to 10-4 mol/L. (3) Cortisol, 17 fyestradiol, or testosterone (10-6 mol/L) also inhibited AVP release within 20 min, but dexamethasone, aldosterone, progesterone, RU486, or cholesterol had no significant effect. (4) RU486 (10-5-10-3 mol/L) could partially block the rapid inhibitory effect of corticosterone. These results suggested that GC might exert in citu a regulatory negative feedback action on the AVP release from hypothalamus of rat through a non-genomic rather than a genomic mechanism.

关 键 词：下丘脑薄片 精氨酸加压素 糖皮质激素 

分 类 号：R338.7[医药卫生—人体生理学] Q426[医药卫生—基础医学]