大鼠中脑腹侧被盖区在睡眠－觉醒调节中的作用  

REGULATORY EFFECT OF VENTRAL TEGMENTAL AREA ON SLEEP-WAKEFULNESS IN RATS

作　　者：张文慧[1] 钱朝霞[1] 江海宏[1] 蔡葵[1] 林殷利[1] 

机构地区：[1]上海第二医科大学生理教研室,海南医学院生理教研室

出　　处：《生理学报》1995年第2期195-200,共6页Acta Physiologica Sinica

摘　　要：本实验在３１只清醒自由行动的雄性ＳＤ大鼠进行。结果如下：（１）双侧中脑腹侧被盖区（ＶＴＡ）微量注射３．３ｎｍｏｌ溴隐亭后第２—３ｈ觉醒时间显著增加（Ｐ＜０．０１）；６．６ｎｍｏｌ溴隐亭有类似效果；０．６６和１．３３ｎｍｏｌ溴隐亭无明显作用。（２）同样方法ＶＴＡ微量注射２ｎｍｏｌ和４ｎｍｏｌＳＣＨ２３３９０后第２—３ｈ觉醒时间明显减少（分别为Ｐ＜０．０１和Ｐ＜０．０５），但注射３．４ｎｍｏｌ舒必利则无效。（３）红藻氨酸（０．３μｇ）双侧ＶＴＡ注后一周，大鼠白天觉醒减少，夜间无明显变化。以上结果表明溴隐亭微量注射到ＶＴＡ可能通过Ｄ１受体使多巴胺神经元活动增加而产生致觉醒作用；另外ＶＴＡ中ＤＡ神经元对于维持日间的觉醒可能有紧张性作用。Experiments were performed on 31 male SD rats. The results were as follows:(1) The time of waking was increased during the second and third hours after microingjection of 3. 3 and 6. 6 nmol bromocriptine into bilateral VTA (P<0. 01 ), but the 1. 33 nmol group was without significant effect. (2) The time of waking was decreased durlug the second and third hours after microinjection of 2 and 4 nmol SCH23390 into VTA (P<0. 01 and P<0. 05 respectively), while 3. 4 nmol sulpiride group was without effect. (3) One week after administration of kainic acid (0. 3 μg), the rats presented less time of waking in the day (P<0. 05), while sleep-wakefulness cycle at night showed no change. The results suggest that bromocriptine could increase wakefulness by aetivating VTA DA neurons via D1 receptor and that the DA neurons might exert a tonic effect for maintaining the wakefulness at the daytime.

关 键 词：中脑腹侧被盖区 睡眠觉醒周期 多巴胺神经元 

分 类 号：R338.63[医药卫生—人体生理学] Q427[医药卫生—基础医学]