大鼠延髓外周橄榄腹外侧核在中枢化学感受中的作用  

ROLE OF THE LATEROVENTRAL PERIOLIVARY NUCLEUS IN THE CENTRAL CHEMORECEPTION OF RESPIRATION IN RATS

作　　者：田国锋[1] 钱梓文[1] 苏清芬[1] 张镜如[1] 

机构地区：[1]上海医科大学生理学教研室

出　　处：《生理学报》1995年第4期327-335,共9页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：本工作采用微量注射、电损毁、电刺激和微电泳的方法，探讨了大鼠延髓外周橄榄腹外侧核（ＬＶＰＯ）是否真正参与中枢化学感受功能。实验在３８只雄性ＳＤ大鼠上进行。结果表明：（１）微量注射酸化人工脑脊液于ＬＶＰＯ可引起隔神经放电活动明显加强。（２）微电泳给予Ｈ＋对ＬＶＰＯ的自发放电单位主要引起兴奋反应，并且，微电泳给予Ｈ＋引起兴奋反应的部分单位也可被腹外侧表面微量注射酸化人ＣＳＦ所兴奋。（３）损毁ＬＶＰＯ后能明显地减弱呼吸中枢对ＣＯ２的反应性。（４）电刺激ＬＶＰＯ，可以使部分ＮＴＳ和ＮＲＡ区的单位放电频率增加，提示ＬＶＰＯ与ＮＴＳ和ＮＲＡ之间存在功能联系。上述结果提示，ＬＶＰＯ可能在中枢化学感受功能中起重要作用。Using the methods of microinjection, microiontophoresis, electrolytic destruction and electric stimulation, the present work was undertaken to investigate whether the lateroventral periolivary nucleus (LVPO) might play a role in the central mechanism of chemoreception on respiration. Experiments were performed on 38 chloralose-urethane anesthetized Sprague-Dawley rats. The results were as follows: (1) Bilateral microinjection of acidified mock CSF into the LVPO region, evoked an enhancement in respiratory activities. (2) Microiontophoretic application of H+ caused mainly excitatory effeets on the spontaneously discharging units in LVPO region, and some of the excitatory units were also responsible to microinjection of acidified mock CSF to the ventrolateral medullary surface. (3) After the electrolytic destruction of bilateral LVPO region in carotid body denervated rats, respiratory response to inhaled CO2 was attenuated significantly.(4) Electric stimulation of LVPO region caused an increase in discharging frequency of some units in the NTS and NRA regions. The above results indicate that LVPO plays a significant role in the central mechanism of chemoreception.

关 键 词：中枢化学感受 橄榄腹外侧核 延髓外周 

分 类 号：R338.3[医药卫生—人体生理学]