血管升压素易化大鼠主动脉弓压力感受器活动  

VASOPRESSIN FACILITATES AORTIC BARORECEPTOR ACTIVITY IN THE RAT

作　　者：马秀英[1] 李洪祥[1] 何瑞荣[1] 

机构地区：[1]河北医学院基础医学研究所生理教研室

出　　处：《生理学报》1995年第4期394-400,共7页Acta Physiologica Sinica

摘　　要：本工作旨在观察血管升压素（ＡＶＰ）对大鼠主动脉弓压力感受器活动的影响。全部实验是在隔离灌流的大鼠主动脉弓──主动脉神经标本上进行的。在２５例标本，所得结果如下：（１）主动脉弓内压（ＩＡＰ）在１３．３ｋＰａ条件下，ＡＶＰ（１０－８ｍｏｌ／Ｌ）使主动脉神经传入放电积分（ＭＮＡ）较对照增加４０±４．７％（Ｐ＜０．０００１）；升降ＩＡＰ时，ＩＡＮＡ随之增减，ＩＡＮＡ－ＩＡＰ关系曲线与对照相比向左上方移位，曲线最大积分值（ＰＩＶ）由６２１±８５增加至７１１±８１％·ｋＰａ－１（Ｐ＜０．０１），但阈压（ＴＰ），饱和压（ＳＰ），工作范围（ＯＲ）和最大斜率（ＰＳ），均无明显变化（Ｐ＞０．０５）；冲洗掉ＡＶＰ后，主动脉神经传入放电基本恢复对照水平。（２）ＩＡＰ在１３．３ｋＰａ条件下，灌流液中加入Ｖ１受体拮抗剂ｄ（ＣＨ２）５Ｔｙｒ－（Ｍｅ）ＡＶＰ（１０－７ｍｏｌ／Ｌ），８—１０ｍｉｎ后再加入ＡＶＰ（１０－８ｍｏｌ／Ｌ），则ＩＡＮＡ不再变化，压力感受器机能曲线无明显移位。（３）在同一保持压条件下，灌流液中分别加入五个不同浓度的ＡＶＰ（１０－１１－１０－７ｍｏｌ／Ｌ），ＩＡＮＡ分别为对照的１９０％，１６０％，１５０％，１３０％，１?Effect of arginine vasopressin (AVP) on the aortic baroreceptor activity of anesthetized rats was studied in 25 aorta arch-aortic nerve preparations. The results obtamed were as follows: (1 ) By perfusing the isolated aortic arch with AVP (10-8 mol/L), at constant intra-aortic pressure (IAP) of 13. 3 kpa, the integrated aortic nerve activity (IANA) was increased by 40f 4. 7% (P<0. 0001 ), and the stepwise increase or decrease of IAP were accompanied correspondingly by changes of IANA. The function curve of aortic baroreceptor was shifted to the left and upward, and the peak integrated value (PIV) of the curve was increased from 621 ± 85 to 711 ± 81 %· kPa-1(P<0. 01 ). There were no changes in threshold pressure (TP), saturation pressure (SP), operating range (OR) and peak slope (PS). After washing out of AVP, the IANA returned to the control level. (2) The above effects of AVP were abolished by pretreatment with V1-antagonist [d (CH2)5Tyr- (Me) AVP, 10-7 mol/L]. (3) By perfusing the aorta-arch preparation with five different concentrations of AVP (10-11 10-7 mol/L), at the same constant IAP, IANA were respectively 190 %, 160 %,150%, 130% and 103% of the control. After pretreatment with V2-antagonist [d (CH2) (d-Ile2Abu4] AVP (10-7 mol/L), the perfusions with five different concentrations of AVP increased the IANA respectively to 130 %, 150%, 160 %, 170% and 180% of the control. When the V2-receptor-mediated vasodilating effect of AVP was blocked, the facilitatory effect of AVP on aortic baroreceptors via V1 receptors was significantly exhibited in a dose-dependent manner. Taken into consideration of the above observations, it is indicated that the V1-receptors-mediated vasoconstricting mechanism may account for the facilitatory effect of AVP on aortic baroreceptors.

关 键 词：管升压素 主动脉弓 压力感受器 主动脉神经 

分 类 号：R338.3[医药卫生—人体生理学]