低氧诱导兔血MDA和棘状红细胞增加及其被MPEG－SOD抑制  

HYPOXIA INDUCED INCREASE AND ECHINOCYTES FROM ERYTHROCYTES IN RABBIT'S BLOOD WITH SPECIAL REFERENCE TO INHBION OF THE INCREASE BY MPEG-SOD

作　　者：韩沾元[1] 陈铭[1] 吕及忍 闻萍[1] 宋雷蕾[1] 吴奇英[1] 

机构地区：[1]中国科学院上海生理研究所,上海医药工业研究院

出　　处：《生理学报》1995年第6期565-572,共8页Acta Physiologica Sinica

基　　金：中国科学院上海生理研究所低氧开放实验室资助

摘　　要：兔模拟海拔高度５０００ｍ低氧（低压舱，ＰａＯ２＝６．９ｋＰａ），按０．２５，０．５，１，２，３，４，５和２４ｈ顺序，红细胞超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＥＣ１．１５．１．１，ＳＯＤ）活性百分数分别下降至８６％，７６％，８１％，８４％，５５％，８１％，８４％和９５％，在０．５和３ｈ酶活性下降显著（与低氧前比，ｎ＝５，Ｐ＜０．０５和Ｐ＜０．０１）。在低氧１，２，３，５和２４红细胞和血浆中两二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）明显增加（与低氧前比，ｎ＝５，Ｐ＜０．０５和Ｐ＜０．０１）。在低氧前由腹腔注入维生素Ｅ（ｖｉｔａｍｉｎｅＥ，ＶＥ），则明显抑制低氧诱导血浆ＭＤＡ增加，但对红细胞ＭＤＡ增加的抑制无统计学意义。这可能与注入的ＶＥ未加载体（ｖｅｈｉｃｌｅ）不能有效渗入红细胞膜内有关。低氧２４ｈ，血液中棘状红细胞（ｅｃｈｉｎｏｃｙｔｅｓｆｒｏｍｅｒｙｔｈｒｏｃｙｔｅｓ）明显增加（与低氧前比，ｎ＝５，Ｐ＜０．０１）。然而在低氧前３０ｍｉｎ由股静脉注入单甲氧基聚乙二醇－ＳＯＤ（ｍｏｎｏｍｅｔｈｏｘｙｐｏｌｙｅｔｈｌｅｎｅｇｌｙｃｏｌ－ｓｏｏ，ＭＰＥＧ－ＳＯＤ），低氧诱导红细胞ＭＤＡ和?When rabbits acutely exposed to hypobaric hypoxia (at imitative altitudeWhen rabbits acutely exposed to hypobaric hypoxia (at imitative altitude 5 000m, PaO2= 6. 7 hpa), the percentage of the activity of superoxide dismutase (SOD in erythrocyte;EC 1.15.1.1 ) decreased respectively to 86%, 76%, 81 % 84%'55%, 81 %,84% and 95% for a Period of 0.25,0.5, 1,2,3,4,5 and 24 h hypoxia while MDA (malondialdehyde) in erythrocyte and plasma increased significantly during hypoxia Periods of 1,2,3,5 and 24 h (n= 5, P<0. 05, P<0. 01 vs. normoxia group).The increase of MDA in plasma could be prevented by vitamin E (V.E,i.p,1 mg daily for 3 consecative days before hypoxia),but that in erythrocyte did not show significant change probably because the injected V.E.without vehicle cannot Penetrate through the erythrocyte membrane effectively.The echinocytes from erythrocytes increased significantly after hypoxia for 24 h (n= 5, P< 0. 05 vs. normoxia group).However,by injecting MPEG-SOD (monomethoxypolythlene glycol-SOD) intravenously 30 min before hypoxia, the increase of MDA (both of erythrocyte and plasma) and echinocytcs (from erythrocytes) were both inhibited obviously during hypoxia for 24 h (n= 5, P<0.05, PRO.of vs.hypoxia group). It is inferred that the increase of MDA and echinocytes from erythrocytes may be due to lipid Peroxidation by superoxide free radicals (O) generated in erythrocytes through Fenton pattern Haber Weiss reaction during hypobaric hypoxia.

关 键 词：低氧 高原 超氧化物歧化酶 丙二醛 棘状红细胞 

分 类 号：R339.54[医药卫生—人体生理学]