螺旋霉素链霉菌噬菌体的分离和鉴定  

ISOLATION AND IDENTIFICATION OF PHAGES ATTACKING S.SPIRAMYCETICUS N.SP.298-36

作　　者：周健明[1] 朱逸忻 陈孝康[1] 刘玉顺[1] 朱建华[1] 沈仁权[1] 盛祖嘉[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《中国抗生素杂志》1989年第4期247-249,共3页Chinese Journal of Antibiotics

摘　　要：从螺旋霉素的异常发酵液及土壤样品中分离到19株螺旋霉素链霉素噬菌体,对每一噬菌体进行了纯化,根据血清中和反应,这些噬菌体可分为4种血清型(P_(11)型、1010型、1012型及4031型)。P_(11)型和1012型噬斑小而清晰,4031型和1010型大而不清晰。观察了4种血清型的代表性噬菌体形态和它们的DNA的限制性内切酶酶切片断长度,多态性。Nineteen cultures of phage attacking S. spiramyceticus were isolated from the abnormal fermentation liquid and soil samples. Phages were purified and classified into four serotype according to the serological neutralization reaction. The phage plaque of 1010 and 4031 were large and translucent, and Pll and 1012 were small and transparent. Representative phages of these form serotypes, namely P11, 1010. 1012 and 4031 were studied maphologically and restriction fragment length polymorphism of their DNA was observed.

关 键 词：螺旋霉素 链霉菌 噬菌体 分离 

分 类 号：TQ465.92[化学工程—制药化工]