脂质体介导的链霉菌染色体DNA转化原生质体的初步研究  

PRELIMINARY STUDY ON THE TRANSFORMATION OF LIPOSOMEMEDIATED CHROMOSOME DNA IN S.FRADIAE

作　　者：罗仲抒 李焕娄[1] 

机构地区：[1]中国医学科学院医药生物技术研究所

出　　处：《中国抗生素杂志》1989年第6期411-414,共4页Chinese Journal of Antibiotics

摘　　要：在新霉素产生菌弗氏链霉菌中作了脂质体包埋染色体DNA转化的实验。对菌株9AS(Arg^-)作受体原生质体,9HC(His^-)作供体DNA来源。在PEG1000存在时,包有染色体DNA的脂质体转化频率较游离DNA高约1个数量级。整个转化过程是抗DNA酶的。本实验的可重复性较差,实验条件还有待进一步改进。The transformation of liposome-entrapped chromosomal DNA was carried out in Str. fradiae, a neomycin producer.At the presence of PEG 1000, the liposomes containing chromosomal DNA of a auxotrophic mutant 9HC(his-) were fused with the protoplasts of another auxotrophic 9AS(arg-).The prototrophic tran-sformants were isolated at a frequency of 10,one magnititude order higher than that of naked DNA. The process of transformation is resistant to DNase.

关 键 词：脂质体 链霉菌 转化 染色体 

分 类 号：TQ465.2[化学工程—制药化工]