绛红色小单孢菌烈性噬菌体Mph1的分离及其特性  

ISOLATION AND CHARACTERIZATION OF A VIRULENT ACTINOPHAGE MPH1 OF M.PURPUREA

作　　者：周健明[1] 朱逸忻 陈孝康[1] 刘玉顺[1] 朱建华[1] 沈仁权[1] 盛祖嘉[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《中国抗生素杂志》1989年第6期408-410,共3页Chinese Journal of Antibiotics

摘　　要：从庆大霉素产生菌——绛红色小单孢72~#异常发酵液中分离到一株烈性噬菌体,能裂解绛红色小单孢菌,形成清晰的噬菌斑,直径2mm左右,侵染指示菌的潜伏期为2小时,增殖期为1.5小时,当pH小于3或大于10及温度高于60℃时明显失活.用限制性内切酶酶解噬菌体发现有EcoRⅠ、HindⅡ、BamHⅠ、SaIⅠ切割位点,DNA分子量约为30kb,电镜观察表明该噬菌体有一六角形头部和一短尾部。A virulent actinophage has been isolated from abnormal fermentation of M. purpurea strain 72. It forms small and tramolucen plaques on indicator strain. The latent period and rise period of one step culture were determinded. It was inactivared at pH beyond 3 and 10 and at tmperature over 60℃. DNA was extracted and digested with restriction endonuclease, EcoR1 HindⅡ BamHi Sali cleavage sites were found; The molecular weight was found to be 3 Kb Microscopic observation revealed a hexagonal head with a short tail.

关 键 词：噬菌体 分离 庆大霉素 单孢菌 

分 类 号：TQ465.2[化学工程—制药化工]