双价核酶对烟草花叶病毒的两个靶序列的专一切割作用  

Di-component Ribozyme Can Function as two Independent Ribozymes on Tobacco Mosaic Virus RNA

作　　者：李洁[1] 金海翎 张庆琪 宋任涛 许政■ 

机构地区：[1]中国科学院上海植物生理研究所植物分子遗传国家重点实验室,上海200032

出　　处：《生物工程学报》1995年第1期20-26,共7页Chinese Journal of Biotechnology

基　　金：国家863高技术基金

摘　　要：报道了两个最简单的多价核酶即双价核酶RZ34和RZ13的构建和体外作用情况。实验结果表明,这两个双价核酶能分别对两个不同的TMV底物或其混合物施行专一切割。双价核酶对单个底物的作用效率与其相应的单价核酶相似。还就双价核酶内的单元核酶的相对位置对其专一性和作用效率的影响进行了探索。In this report, we constructed two di-component ribozymes RZ34 and RZ13, the simplest form of multi-component ribozyme, by ligating two single ribozyme RZ3 and RZ4, and RZ1 and RZ3, respectively. The in vitro results indicated that each two-component ribozyme was able to cleave two different TMV transcripts, with the specificity and efficiency similar to its single ribozyme counterpart. Possible effects on the specificity and efficiency due to the position of the unit-ribozyme in these two-component ribozymes were discussed.

关 键 词：双价核酶 烟草花叶病毒 专一切割 

分 类 号：S432.41[农业科学—植物病理学] Q75[农业科学—农业昆虫与害虫防治]