用微载体技术培养家蚕BmN细胞的实验研究  

Experimental Research of Microcarrier Culturation of Silkworm BmN Cell

作　　者：邓小昭[1] 周永春[2] 张林元[1] 刁振宇[1] 陈宜峰[3] 

机构地区：[1]南京军区军事医学研究所分子遗传学研究室,南京210032 [2]第二军医大学分子遗传学教研室,上海200433 [3]南京师范大学生物系,南京2100243

出　　处：《生物工程学报》1995年第4期332-336,共5页Chinese Journal of Biotechnology

基　　金：江苏省"八五"攻关课题一部分

摘　　要：观察了家蚕ＢｍＮ（从Ｓｉｌｋｗｏｒｍ Ｂｏｍｂｙｘ ｍｏｒｉ获得的细胞系）细胞在微载体Ｃｙｔｏｄｅｘ３上的贴壁分布，提出其分布符合Ｐｏｉｓｓｏｎ规律，并由此估计了不同细胞接种浓度时裸球的百分比，与实际观测结果基本符合；研究了不同接种浓度与微载体浓度时细胞的生长情况，家蚕ＢｍＮ细胞在Ｃｙｔｏｄｅｘ ３上生长的临界接种数为一珠粒６．４个细胞。当微载体浓度为３ｇ／Ｌ时，最低的接种浓度为１．０×１０＾５／Distribution of silkworm BmN cell attachment to Cytodex 3 microcarrier was observed and could be described by Poisson distribution equation. From both empirical results and by application of the poisson equation the proportion of empty beads was shown. Influence of cell inoculation concentration and microcarrier concentration on cell growth was studied here. The critical cell number was determined to be 6. 4 per micro-carrier bead. The minimal cell inoculation concentration was 1.0×105/ml when Cytodex 3 microcarrier concentration was 3g/L.

关 键 词：家蚕 微载体 昆虫细胞培养 

分 类 号：S881.2[农业科学—特种经济动物饲养]