奶牛ZFY、ZFX基因片段的克隆及测序  

Cloning and Sequencing of ZFY and ZFX Gene From Cow.

作　　者：唐榕[1] 马匆匆 毛裕民[1] 徐亚欧[1] 龚荣慈[1] 

机构地区：[1]复旦大学遗传学研究所,西南民族学院牧医系

出　　处：《生物化学与生物物理进展》1995年第1期60-63,共4页Progress In Biochemistry and Biophysics

基　　金：复旦大学遗传工程国家重点实验室资助课题

摘　　要：奶牛的早期胚胎性别鉴定是奶牛胚胎分割及移植技术产生较大经济效益的前提，用ＰＣＲ技术进行早期胚胎性别鉴定具有准确、快速、灵敏度高的特点。利用人和鼠的性别分化相关的ＤＮＡ序列ＺＦＹ、ＺＦＸ基因序列设计的引物对公牛和母牛的染色体ＤＮＡ、ＰＣＲ扩增，将扩增产物定向克隆到ｐＵＣ１１８上，获得ＺＦＹ、ＺＦＸ转化子，并测定了ＺＦＹ、ＺＦＸ基因的序列，发现两者同源性达８８．２％，以此为基础可设计引物和探针，以ＰＣＲ方法进行高灵敏度的奶牛性别鉴定。ex identification of cow embryo in its early stage is very important.It is thepremise of the possibility that the technologyof seperating and transplanting cow embryocan bring about great economic efficacy. PCRtechnology is an accurate, speedy and sensi-tive method in sex identification of cow em -bryo,Usign the human sex differentiation rele-vant DNA sequences ZFY and ZFX,twoprimers are designed and a fragment is ampli-fied from the male or female cow genomicDNA,Then the fragments are cloned into thevector pUC 118 and are sequenced.It is foundthat the fragments from ZFY gene and ZFXgene have 88.2% homologous regions,Uponthis,primers and probes can be designed toidentify the cow sex sensitively by means ofPCR。

关 键 词：乳牛 ZFY基因 ZFX基因 克隆 测序 

分 类 号：S823.2[农业科学—畜牧学]