在96孔板中进行抗脂质过氧化的微量测定  

One-Step Microassay for the Measurement ofAnti-Lipid Peroxidation in 96-Wells Plate.

作　　者：孙士勇[1] 徐波[1] 李秋菊[1] 李润沼[1] 

机构地区：[1]北京医科大学天然药物及仿生药物国家重点实验室

出　　处：《生物化学与生物物理进展》1995年第2期165-169,共5页Progress In Biochemistry and Biophysics

基　　金：国家"八五"科技攻关课题

摘　　要：以Ｆｅ￣（２＋）／半胱氨酸诱导大鼠肝微粒体为基本模型，根据硫代巴比妥酸（ＴＢＡ）反应原理，优化不同反应条件，建立了一种在９６孔板上进行抗脂质过氧化测定的一步反应方法，该方法的灵敏度不低于传统的试管法，而且还具有微量、快速、简便等优点，特别适用于大规模筛选和研究抗氧化剂。此外，也可用于其它系统诱导的抗脂质过氧化的测定。Based on the mode of formation ofmicrosomal lipid peroxdation induced byFe￣(2+)/cysteine and the principle of TBA reac-tion,a simple one-step microassay for thestudying and screening of antioxidants in 96-wells plate was established. The comparativestudies between the procedures in 96-wellsplate and in test tube were also conducted.This microassay requires only small volume ofdiluted microsome and tested compounds,having the advantages of reliability,rapid-ness,simplicity and convenience. It is espe-cially suitable for the pilot screening of antiox-idants as well as the simultaneous study formany samples. This method can also be usedto measure the lipid peroxidation induced byother systems.

关 键 词：微粒体 抗氧化剂 过氧化脂质 药物筛选 

分 类 号：R965.2[医药卫生—药理学]