流式细胞术免疫荧光测量中的直标及间标  

The Direct and the Indirect Immunofluo-rescent Labeling on Immune Measurement ofFlow Cytometry.

作　　者：温厚津 陶家平[1] 

机构地区：[1]北京医科大学分析计算中心细胞分析室

出　　处：《生物化学与生物物理进展》1995年第2期159-162,共4页Progress In Biochemistry and Biophysics

摘　　要：直接标记和间接标记是免疫荧光测量中两种不同的标记方法。在流式细胞计测量中，一般说来，直接标记不易引入样品的非特异性结合，因此引入干扰少，样品的数据好处理，而间接标记由于有放大作用，容易引入非特异结合的干扰，严重时所得数据无法区分特异及非特异信号，造成数据分析的混乱，通过样品实测比较，认为在流式细胞计测量中应用直接样品更为合适。The direct and the indlrect fluores-cent labeling are two different staining meth-ods in immunofluorescent measurement.Inflow cytometer measurement,in general,thedirect labeling has little nonspecific bindingand disturbance,and the experiment data iseasy to process,However,because of themagnifying function,the indirect labeling hasmuch nonspecific binding and serous distur-bance. It is difficult to distinguish the specificsignals from non-specific ones,it is easy tocause confusion in data processing. By com-paring samples,the direct labeling issuggested better than the indirect labeling inflow cytometer measurement.

关 键 词：直接标记 间接标记 流式细胞计 免疫荧光测量 

分 类 号：R392－33[医药卫生—免疫学]