大脑皮层神经元膜蛋白构象改变的ESR研究  

ESR Study on Conformation of MembraneProteins of CerebraI Cortex Neurons.

作　　者：章茜[1] 吴本[1] 于桂芬[1] 卢景雰[1] 

机构地区：[1]北京医科大学生物物理系,河南医科大学生理教研室,北京医科大学天然药物和仿生药物国家重点实验室

出　　处：《生物化学与生物物理进展》1995年第4期337-340,共4页Progress In Biochemistry and Biophysics

基　　金：国家"八五"攻关课题

摘　　要：采用马来酰亚胺标记完整的大脑皮层细胞，观察由低氧引起的ＥＳＲ谱线的变化及脑细胞脂质过氧化程度，低氧引起细胞过氧化物生成增加，膜蛋白构象改变．金属硫蛋白（１０￣（－５）ｍｏｌ／Ｌ）能明显抑制过氧化反应，具有一定的抗氧化作用．并对实验的分子机理进行了简要的讨论．Maleimide was used as spin labelfor studying the effect of hypoxia on rat cere-bral cortex neurons.Results showed that hy-poxia induced the lipid peroxidation in cerebralcortex cells and increased the formation ofLPO.By comparing the characteristics of ESRspectra,it was found that values of τ_c weresignificantly higher in hypoxic group than thatin control(P<0. 01).The increase in the ra-tio of*h_o/h_(-1) was well correlated with the en-hance in hypoxia group. It was also found thatMT could inhibit the lipid peroxidation incerebral cortex cells.

关 键 词：大脑皮层 神经元 膜蛋白 构象 电子自旋共振 

分 类 号：Q593.2[生物学—生物化学]