大鼠脊髓细胞膜胰岛素受体的结合特性  

Binding Properties of Insulin Receptor in Rat Spinal Cord Cell Membrane

作　　者：朱尚权[1] 徐明华[1] 张新堂[1] 叶莺[1] 姜新建[1] 林淑琼[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海市第一人民医院康复科

出　　处：《生物化学与生物物理学报》1995年第1期111-115,共5页

基　　金：国家自然科学基金

摘　　要：大鼠脊髓细胞膜胰岛素受体的结合特性朱尚权，徐明华，张新堂，叶莺（中国科学院上海生物化学研究所，２０００３１）姜新建，林淑琼（上海市第一人民医院康复科，２０００８５）关键词胰岛素受体，脊髓细胞膜免疫组织化学、放射免疫自显影和放射受体测定技术已证明脑各区...Insulin receptor binding properties have been studied in rat spinal cerd cell membrane.It was shown that the binding of 125I-insulin to spinal cord cell membrane was time dependent, reaching equilibrium after 20 hours of incubation at 4℃ with optimal pH7.8.The specific and nonspecific binding were 2.79% and 0.83% respectively.This binding was reversible,saturable and specific.Scatchard analysis showed that the dissociation constant (Kd) and binding capaeity (Bc) for high affinity binding sites were 6.26×10-8M and 2.1 pmol/mg protein,respectively,and the Kd and Bc for low affinity sites were 7.0×10-6M and 72.3 pmol/mg protein,respectively.

关 键 词：胰岛素 受体 脊髓 细胞膜 

分 类 号：Q578[生物学—生物化学]