LA－90细胞在温度转化过程中蛋白质酪氨酸磷酸化作用研究  

Changes in Tyrosine Protein Phosphorylation and Dephosphorylation in ts-RSV LA-90 Cells Transformation

作　　者：夏英[1] 高漫[1] 颜卉君[1] 吴国利[1] 

机构地区：[1]北京师范大学生物系生物化学及分子生物学研究室

出　　处：《生物化学与生物物理学报》1995年第2期223-227,共5页

基　　金：国家自然科学基金

摘　　要：ＬＡ－９０细胞在温度转化过程中蛋白质酪氨酸磷酸化作用研究夏英，高漫，颜卉君，吴国利（北京师范大学生物系生物化学及分子生物学研究室，１００８７５）关键词酪氨酸蛋白激酶；磷酸酪氨酸蛋白磷酸酶；细胞转化ｉｓ－ＲＳＶＬＡ－９０细胞是ＲＳＶ转染的小鼠３Ｔ３细胞...ts-RSV LA-90 cell line is a temperature-sensitive mutant of the RSV transformed Swiss 3T3 cell line.When cultured at 39℃ it is in normal state and at 33℃ it is in transformed state.In our experiment the membrane-associated(particulate fraction) and cytosolic(soluble fraction) tyrosine protein kinase activities and the phosphorylated level of endogeous substrates of transformed state are higher than those in normal state and phosphotyrosine protein phosphatase activities of transformed state are lower than the activities of normal state during the cultured time observed.We also found that the changes of tyrosine protein kinase activities and phosphotyrosine protein phosphatase activities with the cultured time were fluetuating and wore similar both in normal state and in transformed state.So was the level of tyrosine protein phosphorylation.The greatest change is in 8-hours.This reflects the rhythm of cell control processes.

关 键 词：酪氨酸蛋白激酶 磷酸酪氨酸蛋白磷酸酶 细胞转化 

分 类 号：Q26[生物学—细胞生物学]