含硒抗体酶性质的研究  

Studies on the Properties of Se-containing Abzyme

作　　者：朱振齐[1] 罗贵民[1] 丁兰[1] 高贵[1] 孙启安 杨同书[1] 沈家骢[1] 

机构地区：[1]吉林大学酶工程国家重点实验室,白求恩医科大学地方病研究所,吉林大学分子光谱和结构开放实验室

出　　处：《生物化学与生物物理学报》1995年第4期431-435,共5页

摘　　要：在成功地制备了具有谷胱甘肽过氧化物酶（ＧＰＸ）活性含硒抗体酶（Ｓｅ－ａｂｚｙｍｅ）的基础上，我们筛选了制备Ｓｅ－ａｂｚｙｍｅ的最佳条件，并对其理化性质及酶学性质和稳定性进行了深入的研究。结果表明，Ｓｅ－ａｂｚｙｍｅ的等电点为６．９５和７．０８，分子量为１５８ｋｄ；最适ｐＨ和最适温度范围比天然酶宽广；抗体酶的贮藏稳定性比天然酶高。用Ｘ射线光电子能谱技术测得在Ｓｅ－ａｂｚｙｍｅ中含硒量为５ｍｏｌＳｅ／ｍｏｌ。用酶联免疫法测得Ｓｅ－ａｂｚｙｍｅ对谷胱甘肽（ＧＳＨ）的结合常数为１．１１×１０７（ｍｏｌ／Ｌ）－１。二硫代双硝基苯甲酸（ＤＴＮＢ）可抑制Ｓｅ－ａｂｚｙｍｅ，抑制常数为９×１０－２（ｍｏｌ／Ｌ）。We successfully prepared the Se-containing abzyme with GPX activity and further studied its physicochemical and enzymci properties and stabilities. Data showed that the isoelectric point of the abzyme was 6.95 and 7.08,and its molecular weight was 158kd.The ranges of optimum pH and temperature of the Se-containing abzyme were wider than those of the native GPX. The stability of the abzyme in storage was higher than that of GPX. The content of Se in the abzyme was found to be 5 mol Se/mol abzyme by X-ray photoelectron spectrum and the binding constant was 1.11×107M-1 as assayed by ELISA method.The Seabzyme was inhibited by dithiobis (2-nitrobenzoic acid),and inhibition constant was determined to be 9×10-2M-1.

关 键 词：含硒抗体酶 抗体酶 性质 

分 类 号：Q550.2[生物学—生物化学]