猪精子凝集素的纯化、性质及其作用  

Purification, Characterization and Effect of Boar Sperm Lectin

作　　者：赵明[1] 费坚[1] 孙册[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1995年第4期347-354,共8页

基　　金：中国科学院七五生物学重大项目美国RockefellerFoundationGrantRF88045资

摘　　要：用胎球蛋白－Ｓｅｐｈａｒｏｓｅ亲和层析和凝胶过滤层析从精子和精浆中分离纯化了猪精子凝集素（简称ＢＳＬ）。ＢＳＬ的血凝活性只被若干糖蛋白和聚精所抑制。ＢＳＬ的分子量为５６ｋｄ，由分子量分别为１３．６ｋｄ（β）和１６．０ｋｄ（α）的两个不同的亚基以α１β３所组成。ＢＳＬ为糖蛋白，含中性糖３．２％，不含唾液酸。用ＥＬＩＳＡ法测定猪精子中ＢＳＬ的含量及分布，表明７０％嵌入在精子膜中，２５％结合在精子表面。胞浆中很少。经酶联结合实验测定表明ＢＳＬ与ＺＰ３结合，亦与精子蛋白质结合。由此设想ＢＳＬ可能在猪精卵结合中起＂桥梁＂作用。A substance which agglutinated rabbit red blood cells has been isolated and purified by fetuin-Sepharose affinity chromatography and gel filtration from boar sperm and sperm plasma.It is denoted as boar sperm lectin(BSL).Its hemagglutinating activity is inhibited only by some glycoproteins and polysaccharides.The molecular weight of BSL is 56 kd.It is a tetramer,α1β3 which consists of 2 subunits,with one 16 kd(α)and three 13.6 kd(β)polypepeides.It is a glycoprotein containing 3.2%neutral sugar without sialic acid. The content and distribution of BSL in boar sperm have been deterined by the ELISA method.It shows that70%BSL is integrated into the sperm membrane, 25% is bound to the glycoproteins on the cell surface,and very little is found in the cell plasma. The binding of BSL with ZP3 and sperm proteins has been determined by IZPBA and Western blot technique respectively.BSL binds with both ZP3 and boar sperm proteins,thus,this could be proposed that BSL may serve as a 'bridge' in sperm-oocyte binding.Further work is in progress to verify this postulation.

关 键 词：猪精子凝集素 提纯 性质 猪 

分 类 号：Q959.842[生物学—动物学]