钙过负荷和丹参酮对线粒体脂质过氧化的影响  

Effect of Calcium Overload and Salviol (Tanshinone)on the Lipid Free Radicals Generated from LipidPeroxidation of the Mitochondrial Membrane

作　　者：赵燕[1] 江文[1] 侯京武[1] 赵保路[1] 忻文娟[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《生物化学与生物物理学报》1995年第6期610-615,共6页

基　　金：国家自然科学基金

摘　　要：用ＥＳＲ自旋捕集技术捕捉到Ｆｅ２＋启动的心肌线粒体膜脂质过氧化过程中产生的脂类自由基Ｌ·（ａＮ＝１５．６Ｇ，ａＨ＝２．９７Ｇ）。钙过负荷时，钙离子使线粒体产生的脂类自由基增加，当钙浓度为３０μｍｏｌ／Ｌ·ｍｇＰｒ时，产生的脂类自由基约增加３２％。丹参酮可清除Ｆｅ３＋启动的线粒体膜脂质过氧化过程中产生的脂类自由基。当丹参酮浓度为０．８ｍｇ／ｍｇＰｒ时，清除率达到７０％左右。钙过负荷时丹参酮仍能有效清除脂类自由基。另外，还发展了用自旋探针ＣＴＰＯ测量线粒体氧消耗的方法，检测氧消耗所需样品量比Ｃｌａｒｋ电极少几十倍。用此方法测量小鼠心肌线粒体不同状态的氧消耗和呼吸控制率，证明一定浓度的Ｃａ２＋和Ｆｅ２＋影响线粒体的呼吸功能．Applying ESR spin trapping technique, we got the spectrum of the lipid radicals generated from the lipid peroxidation of the mitochondrial membrane initiated by ferrous ion. When the [Ca2+] was 30μM/mg Pr, the lipid free radicals increased about 32% compared with the control. The salviol (Tanshinone) is a good scavenger of the lipid free radicals. When its concentration was 0. 8 mg/mg Pr, the scavengingeffeot approximated to 70%. The method of spin probe oxymeter was developed by us to study the oxygen consumption of mitochondria. When the concentration of Ca2+ or Fe2+ increased to a certain degree, the NADH-dependent oxygen consumption of mitochondria acal blocked. From 0.4μg/ml to 0.4 mg/ml, salviol had no protective effect on the function of the respiration of mitochondria injured by calcium or Fe2+ in high concentration.

关 键 词：线粒体 丹参酮 钙离子 过氧化脂质 

分 类 号：Q731[生物学—分子生物学]