1．9A分辨率Al－（L－丙氨酸）胰岛素晶体结构研究  

The Crystallographic Studies of Al-(L-Ala)Insulin Crystal at 1.9 Resolution

作　　者：万柱礼[1] 张公义 常文瑞[1] 梁栋材[1] 

机构地区：[1]中国科学院生物物理所,生物大分子国家重点实验室

出　　处：《生物化学杂志》1995年第1期40-43,共4页

基　　金：国家自然科学基金;中国科学院重大项目基金

摘　　要：使用Ｘ－ＰＬＯＲ及立体化学制约最小二乘精化技术，并结合差值Ｆｏｕｒｉｅｒ图人工分析，测定了１．９　分辨率Ａｌ修饰丙氨酸胰岛素的晶体结构，晶体空间群为Ｒ３，晶胞参数：ａ＝ｂ＝８０．８９，ｃ＝３７．６４。精化后的结构模型最终偏离因子Ｒ＝０．１８５，同理想键长和键角的均方根偏差分别为０．０１８和３．５°，独立区内二个分子的Ａ链Ｎ端Ａｌ－丙氨酸残基清晰可见。The crystal structure of Al-(L-Ala) insulin (Al-L-AlaI) was determined at 1.9A resolution.It belongs to the trigonal system with space group R3.The molecular model was adjusted and refined by a X-PLOR and stereochemically-restrained least squares program,assisted by manual revision of the model based on difference Fourier map.The final R-factor of AL-L-AlaI structure at 1.9A resolution is 0.185 with r.m.s.deviation of 0.020A from the ideal bond lengths and r.m.s.deviation of 3.50°from the ideal bond angles.The results show that the structural model of Al-L-AlaI fitted well to the electron density maps.The main and side chain of two Al-L-Ala residues in the asymmetric unit are ordered.

关 键 词：晶体 三维晶体结构 铝-L-丙氨酸 胰岛素 

分 类 号：Q578.01[生物学—生物化学]