大熊猫乳酸脱氢酶同工酶H_4的分离纯化和某些性质的研究  

Separation and Some Characteristics of LactateDehydrogenase Isozyme H_4 of Giant Panda

作　　者：彭浪[1] 张龙翔[1] 

机构地区：[1]北京大学生命科学学院

出　　处：《生物化学杂志》1995年第1期85-90,共6页

摘　　要：采用８－（６－氨己基）－氨基－５＇－ＡＭＰＳｅｐｈａｒｏｓｅ亲和层析法和ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ离子交换层析法从大熊猫心肌中分离纯化出了乳酸脱氢酶同工酶Ｈ４．纯化的大熊猫ＬＤＨ－Ｈ４，比活为４４５Ｕ／ｍｇ蛋白，经ＳＤＳ－ＰＡＧＥ，ＰＡＧＥ，等电聚焦电泳鉴定均为一条带，其亚基分子量为３６０００，等电点为５．４５．经测定大熊猫ＬＤＨ－Ｈ亚基Ｎ端被封闭，Ｃ端氨基酸残基经测定为Ｌｅｕ．氨基酸组成分析表明每个亚基含有５个Ｃｙｓ，９个Ｍｅｔ．Lactate dehydrogenase (LDH EC 1.1.1.27) isozyme H4 was separated and purified from giant panda heart muscle by 8-(6-aminohexyl)-amino-5’-AMPSepharose 4B affinity chromatography and DEAE-Sepharose ion exchange chromatography. 34mg LDH-H4 were obtained from 100g heart muscle. Its specific activity was 445U/mg. The purified LDH-H4 was shown to be homogenous by SDS-PAGE, PAGE and IEF with a pI of 5.45. The subunit has a molecular weight of 36kD. There are 5 Cys and 9 Met in each subunit according to its amino acid compositon analysis.It has a blocked N-terminal and its C-terminal residue is Leu.

关 键 词：大熊猫 乳酸脱氢酶 同功酶 分离 提纯 

分 类 号：Q959.838[生物学—动物学] Q550.3