模拟谷胱甘肽过氧化物酶活性三肽的制备及其性质研究  

Studies on Preparation and its Properties of Tripeptide with Activity of GPX

作　　者：朱振齐[1] 罗贵民[1] 丁兰[1] 孙启安 杨同书[1] 沈家骢[1] 

机构地区：[1]吉林大学酶工程国家重点实验室,白求恩医科大学地方病研究所,吉林大学分子光谱和结构开放实验室

出　　处：《生物化学杂志》1995年第3期338-342,共5页

摘　　要：开发一种制备硒代谷胱甘肽（ＧｓｅＨ）的新方法。以合成的谷氨酰－γ－丝氨酰－甘氨酸（Ｇｌｕ－Ｓｅｒ－Ｇｌｙ）三肽为原料，经苯甲基磺酰氟（ＰＭＳＦ）活化，用Ｈ＿２Ｓｅ突变Ｓｅｒ成硒代半脱氨酸（ＳｅＣｙｓ）制成ＧＳｅＨ。用元素分析及氨基酸分析确定此三肽的组成并推导出此三肽的结构。研究了ＧＳｅＨ的性质．结果表明，此三肽具有谷胱甘肽过氧化物酶（ＧＰＸ）的活性，其活力比其它一些小分子有机模拟物高，在性质上也有优于它们的一些特点，其动力学行为与天然ＧＰＸ类似。A new strategy for generating GSeH was developed. The serine residue in Glu-γ-Ser-Gly was activated with phenylmethyl-sulfonylfluoride(PMSF),then these residues were converted into selenocysteine by hydrogen selenide (H_2Se) resulting in formation the GSeH. The structure of the tripeptide was demonstrated by the elemental and amino acid analysis. Data showed that the GSeH tripeptide possessed GPX activity. The activity of GSeH was higher than that of other low molecular mimics. The GSeH has an advantage over other mimics in some respects.Study on kinetic property showed that both the GSeH and GPX exhibit similar kinetic behavior.

关 键 词：谷胱甘肽 过氧化物酶 模拟三肽 制备 

分 类 号：R392－33[医药卫生—免疫学]