大鼠脑α_1型甲状腺激素受体基因的cDNA克隆及序列分析  

The cDNA Cloning and Sequencing of the Thyroid Hormone Receptor gene from Rat Brain

作　　者：赵崇[1] 宋学文[1] 张连英 邓彤[1] 张镜宇[1] 

机构地区：[1]天津医科大学内分泌研究所

出　　处：《生物化学杂志》1995年第4期392-395,共4页

基　　金：国家自然科学基金

摘　　要：使用ＲＴ－ＰＣＲ方法克隆了Ｗｉｓｔａｒ大鼠脑α_１型甲状腺激素受体的ｃＤＮＡ，得到包含起始及终止密码子共１２３３ｂｐ、编码４０９个氨基酸的受体全长编码序列．酶切分析后，将此特异ＤＮＡ片段重组入质粒ｐＵＣ系统，得重组质粒ｐＴＲＡ．双脱氧末端终止法测定了全部核苷酸顺序，结果与文献报导的ＳＤ大鼠的结果一致，同时对长片段ＤＮＡ的ＲＴ－ＰＣＲ扩增进行了方法学的探讨。It was cloned that the cDNA of α 1 isoform of thyroid hormone receptor by RT-PCR,a DNA fragment of 1233 bp including the initial and terminal codons. After restrictive enzyme analysis,the PCR product was cloned into the plasmid PUC and subcloned for DNA sequncing by the dideoxy chain termination method. The result was the same with that of Thompson. The methodology of RT-PCR DNA amplification for large gene fragment was also explored.

关 键 词：甲状腺激素 受体 分子克隆 CDNA 序列分析 

分 类 号：Q572[生物学—生物化学]