表皮生长因子，生长抑素对人早孕胎盘hCG分泌及基因表达的影响  

Effect of EGF,SS on hCG Secretion and hCGβ-mRNA Expression in Early Placenta

作　　者：陈敏[1] 时宇[1] 史凌云[1] 李潭[1] 赵玲[1] 

机构地区：[1]北京医科大学生殖医学研究与培训中心,兰州医学院第一附属医院,北京师范大学生物系系,国家计划生育委员会科学与技术研究所

出　　处：《生物化学杂志》1995年第4期483-486,共4页

摘　　要：观察了表皮生长因子（ＥＧＦ），生长抑素（ＳＳ）对体外培养的人早孕绒毛膜促性腺激素（ｈＣＧ）分泌及ｈＣＧβ－ｍＲＮＡ含量的影响。发现ＥＧＦ可明显刺激绒毛分泌ｈＣＧ，显著增加ｈＣＧβ－ｍＲＮＡ含量，生长抑素虽然对绒毛ｈＣＧ分泌及ｈＣＧ　β－ｍＲＮＡ含量无明显影响，但可抑制ＥＧＦ，ＧｎＲＨ刺激的ｈＣＧ分泌及ｈＣＧβ－ｍＲＮＡ水平。提示ＥＧＦ，ＳＳ在妊娠早期参与了ｈＣＧ分泌的调节。Human chorionic gonadotrphin (hCG) is the hormone primarily responsible for maintenance of corpus lutem in early pregnancy. Recently,human placenta has been demonstrated an important source of various neurohormones, growth factors and cytokines. Many evidences showed that the placenta produced the somatostatin (SS) and epidermal growth factor(EGF). In our studies, by using tissue culture and Northern blotting techniques,the effect of SS and EGF on hCG secretion and hCGβ-mRNA expression in human early placenta in vitro was investigated.hCG secretion and hCGβ-mRNA expression could be increased by EGF (50ng/ml) or GnRH (10mol/L) significantly. Compared with the control, hCGβ-mRNA level increased more than 1. 56 or 2. 51 times (P<0. 01) . SS (1000ng/ml) has no effect on hCG basal secretion and hCGβ-mRNA level ,however,it can suppress the effect of EGF or GnRH-stimulated hCG secretion and hCGβ-mRNA expression. This indicates that SS,and EGF may play a role in hCG secretion and hCGβ-mRNA expression in early pregnancy.

关 键 词：HCG 生长因子 生长抑素 基因表达 

分 类 号：Q579.13[生物学—生物化学]