鲤鱼（Cyprinus　carpio）生长激素基因的亚克隆及其序列分析  

Subcloning and Sequencing of Growth Hormone Gene for Carp (Cyprinus carpio)

作　　者：赵晓祥[1] 杨学成[1] 李晶[1] 李莹[1] 张淑梅[1] 杨志兴[1] 

机构地区：[1]黑龙江省科学院应用微生物研究所

出　　处：《生物技术》1995年第2期10-12,共3页Biotechnology

基　　金：黑龙江省科委"八五"攻关课题

摘　　要：本文对ＰＣＲ扩增的６６８ｂｐ的ＤＮＡ片段进行了亚克隆，，然后以Ｓａｎｇｅｒ双脱氧中止法为原理，利用美国ＡＢＩ公司３７０Ａ自动核酸序列分析仪，确定了６６８ｈＰ的核苷酸序列。序列分析表明鲤鱼生长激素基因的开放读框含有６３０ｂｐ，并推测其中包括２２个氨基酸的信号肽和１８８个氨基酸的成熟多肽。鲤鱼生长激素基因的酶切图谱和序列分析的结果都证明我们已获得了全长的鲤鱼生长激素基因。A DNA fragment with EcoR I and Sal I site by reverse transcription and PCR amplification from carp pituitary gland is subcloned in PGEM plasmid. With ASI 370A autosequencer, 668 base pairs of nucleotide sequence of the DNA fragment have been determinedusing the dideoxy chain termination method. Computer aided analysis shows and open reading frame of carp growth hormone gene contains 630 base pairs which include 22 amino acidsof the signal peptide and 188 amino acids of the mature hormone. The result of the restriction map and sequence analysis for carp growth hormone gene indicates. We have obtainedbull--length carp growth hormone gene.

关 键 词：鲤鱼 生长激素基因 亚克隆 DNA 序列分析 

分 类 号：Q959.468[生物学—动物学]