显微红外研究巨噬细胞膜电融合后膜蛋白二级结构的变化  

STUDY ON THE CONFORMATIONAL CHANGES OF MEMBRANE PROTEIN AFTER ELECTROFUSION OF MACROPHAGE MEMBRANE USING MICOR-FTIR

作　　者：沈子威[1] 齐娟娟[1] 张志诚[1] 杨重山[1] 孙素琴[1] 赵南明[1] 苏雅娴 

机构地区：[1]清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室,北京医科大学细胞生物学系

出　　处：《生物物理学报》1995年第2期179-184,共6页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：首次尝试了将显微傅里叶变换红外光谱术应用于研究电融合后细胞膜蛋白质二级结构的变化，发现脉冲电场作用于细胞具有穿透效应，施加电脉冲后，整个细胞的蛋白质体系能量增加，表明电脉冲对蛋白质的二级结构影响很大；同时，还发现用唾液酸苷酶和蛋白酶Ｐｒｏｎａｓｅ分别处理巨噬细胞膜表面后，膜上蛋白质二级结构无序化程度增加，用酶适度处理的细胞将更易发生电融合。Conformational changes of Secondary structure of membrane proteins after membrane electrofusion were investigated using micro-fourier transform inflared spectroscopy. The FTIR results present that the percentage of α-helix strcture in the membrane protein increase and the of β-pleatal sheet in the membrane protein decreases after the electrofusion of MΦ membranes. It suggests that the membrane fusion of MΦ may improve its bioacivity. These show that pulsed electric field has permeation effect on cells, it increases the total energy of Cell protein system. and has remarkable influence on Secondary structure of membrane proteins. Incubated with salic adenalytase and Pronase E respctively, the disordering of Secondary structure of membrane proteins increase, showing that it will be easier for MΦ cells to perform electrofusion after incubated with enzymes.

关 键 词：显微红外 巨噬细胞 电融合 膜蛋白质 二级结构 

分 类 号：Q271[生物学—细胞生物学]