细胞色素bc_1复合物的喇曼光谱研究  

STUDY ON CYTOCHROME bc_1 COMPLEX WITH RAMAN SPECTROSCOPY

作　　者：朱克莉[1] 张亦昕[1] 徐建兴[1] 何季平[1] 岳文海[1] 解荣[1] 

机构地区：[1]中国科学院生物物理研究所,武汉工业大学分析测试中心

出　　处：《生物物理学报》1995年第2期144-148,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金;中国科学院项目

摘　　要：对提纯的细胞色素ｂｃ１复合物的氧化态和底物琥珀酸还原态两个样品进行了共振喇曼和富立叶喇曼光谱测定和比较。琥珀酸还原态与氧化态的共振谱比较明显有变化，而富立叶红外谱没有什么差别。说明呼吸链的电子传递体在氧化态与还原态交替变化进行电子传递时，蛋白总体构象不发生大的改变，而活性中心血红素辅基局部构象变化很大。The purified cytochrome bc1 complex was studied by comparing their resonance Raman (RR-Raman) spectra and Fourier Transformed Raman (FT-Raman) spectra in its oxidized and reduced states. The RR-Raman spectra of reduced and oxided bc1 complex were extremely different, while the FT-Raman spectra did not change much with their rebox states.These results indicated that the local conformation of heme group but not the comformation of whole protein molecular changed with their redox states.

关 键 词：细胞色素 bc1复合物 共振喇曼 喇曼光谱 

分 类 号：Q559.902[生物学—生物化学]