高峰淀粉酶的分子可及性的研究  

A STUDY ON THE MOLECULAR ACCESSIBILITY OF TAKA-AMYLASE A

作　　者：汪大伟[1] 林大威[1] 张学忠[1] 吴晓霞[1] 丁忠田 

机构地区：[1]东北师范大学物理系,北京大学化学系,吉林大学酶工程实验室

出　　处：《生物物理学报》1995年第2期134-138,共5页Acta Biophysica Sinica

摘　　要：依据高峰淀粉酶的晶体结构数据对它的分子可及性进行了计算和分析，得到了极性与非极性氨基酸的分布、极性原子与非极性原子对可及性的贡献、催化活性中心裂隙的构象、Ｃ末端结构域空间拓扑等信息。活性部位氨基酸残基的可及性研究结果与酶－底物复合物模型相符。In this paper, the molecular accessibility of Taka-Amylase A (TAA) was calculated and analyzed on the basis of its crystal structure data. Some useful information, such as the distribution of polar and non-polar amino acids in surface and core of the molecules, the comformation and function of the catalytic cleft, the folding topology of the C-terminal domain, were obtained. The result of the accesibility analysis of the residues in the active center tallied with the enzyme-substrated complex model.

关 键 词：分子可及性 高峰淀粉酶 淀粉酶 

分 类 号：Q556.202[生物学—生物化学]